IN THIS STUDY, WE EVALUATED THE PHOTOCATALYTIC EFFICACY OF PURE Y2TI2O7 FILMS INCORPORATING VARYING AGO RATIOS (1.0, 3.0, AND 5.0 MOL%) IN THE DEGRADATION PROCESS OF BRILLIANT GREEN DYE AND IN THE BACTERIAL DISINFECTION OF ESCHERICHIA COLI UNDER UV?VISIBLE ILLUMINATION. THE FILMS WERE PREPARED UTILIZING A PHOTOCHEMICAL METHODOLOGY INVOLVING ?-DIKETONATE COMPLEXES OF Y(DBM)3, TI(DBM)4, AND AG(ACAC) AS STARTING MATERIALS. FOLLOWING SYNTHESIS, THE PHOTO-DEPOSITS UNDERWENT CALCINATION AT 900 °C FOR 2 H AND WERE SUBSEQUENTLY ANALYZED USING FOURIER TRANSFORM INFRARED SPECTROSCOPY, X-RAY DIFFRACTION, SCANNING ELECTRON MICROSCOPY - ENERGY DISPERSIVE X-RAY SPECTROSCOPY, AND X-RAY PHOTOELECTRON SPECTROSCOPY. THE OUTCOMES OF THESE CHARACTERIZATIONS INDICATE THE FORMATION OF A PYROCHLORE Y2TI2O7 CUBIC PHASE, WITH THE EMERGENCE OF AGO IN THE DOPED SAMPLES. PHOTOCATALYTIC PERFORMANCE YIELDED SIGNIFICANT RESULTS, WITH A 94.9 % DEGRADATION OF BRILLIANT GREEN OBSERVED FOR Y2TI2O7 SAMPLES DOPED AT 3.0 MOL%, AND A 98.4 % INHIBITION OF ESCHERICHIA COLI CULTURES OBSERVED FOR Y2TI2O7 SAMPLES DOPED AT 1.0 MOL%. THEORETICAL CALCULATIONS WERE EMPLOYED TO DETERMINE THE BAND EDGE POTENTIALS OF BOTH OXIDES, SUPPORTING THE ESTABLISHMENT OF A TYPE I HETEROJUNCTION BETWEEN THE Y2TI2O7 PHASE AND AGO. A DEGRADATION MECHANISM HAS BEEN POSTULATED BASED ON ASSAYS INVOLVING THE BLOCKING OF REACTIVE OXYGEN SPECIES AND CHARGE CARRIERS THROUGH THE USE OF SPECIFIC

AMARYLLIDACEAE PLANTS ARE WELL KNOWN FOR PRODUCING ISOQUINOLINE-TYPE ALKALOIDS WHICH HAVE IMPORTANT BIOLOGICAL ACTIVITIES, HOWEVER THE PRODUCTION OF THESE SECONDARY METABOLITES IN PLANTS IS VERY LOW, IT?S NECESSARY TO STUDY MECHANISMS TO INCREASE THE PRODUCTIVITY OF THEM. OUR STUDY SHOWS THE ALKALOIDS COMPOSITION ON THE WILD BULBS AND IN VITRO BULBS OF FOUR SPECIES CHILEAN AMARYLLIDACEAE. QUALITATIVE AND SEMI-QUANTITATIVE ANALYSIS OF ALKALOID EXTRACTS CARRIED OUT BY GC/MS?MS WE FOUND A TOTAL OF 48 DIFFERENT ALKALOIDS PRODUCED IN WILD PLANTS AND IN VITRO CULTURE; 38 ALKALOIDS WERE IDENTIFIED. THESE RESULTS SUGGEST THAT DIFFERENT COMBINATIONS OF AUXINS AND CYTOKININS CAN MODULATE PLANT BIOCHEMICAL PATHWAYS THAT REGULATE ALKALOID BIOGENESIS AND SHOW THE HIGH POTENTIAL THAT PLANTS OF THE AMARYLLIDACEAE FAMILY HAVE TO PRODUCE LYCORINE TYPE ALKALOIDS. THIS STUDY ALLOWED KNOWING THE CONTENT OF ALKALOIDS IN WILD AND IN VITRO BULBS OF THE STUDIED SPECIES. THESE RESULTS ENABLE TO ESTABLISH THE CULTURE MEDIA WITH 4.44 ?M BAP AND 2.70 ?M NAA AND 2.20 ?M BAP AND 5.40 ?M NAA INCREASED THE PRODUCTION OF LYCORINE-TYPE ALKALOIDS FOR R. ADVENA AND R. PRATENSIS RESPECTIVELY, THEREFORE IT IS ABLE TO PROMOTE OXIDATIVE PHENOL COUPLING VIA ORTHO-PARA' AND DEMONSTRATES HOW IT IS POSSIBLE TO DIRECT THE PRODUCTION OF THESE MOLECULES THROUGH THE USE OF PHYTOHORMONES IN IN VITRO CULTURE. FINALLY, WE CAN SAY THAT THE RESULTS CONFIRM THAT MICROPROPAGATION IS A GOOD TOOL TO PRODUCE THIS TYPE OF ALKALOIDS.

THE MISUSE OF ANTIBIOTICS HAS LED TO HIGH LEVELS OF DRUG-RESISTANCE IN SPECIFIC PATHOGENS, PROMOTING THE SEARCH OF MOLECULES FROM DIFFERENT NATURAL SOURCES OR THE DESIGN OF NOVEL DRUG CANDIDATES. MEDICINAL AND EDIBLE PLANTS ARE A RICH SOURCE OF BIOACTIVE COMPOUNDS, IN PARTICULAR THOSE OF POLYPHENOL CLASS. IN THE PRESENT WORK, WE SCREEN THE ANTIMICROBIAL PROPERTIES OF AN AQUEOUS EXTRACT PREPARED FROM THE LEAVES OF UGNI MOLINAE (TURZ) AGAINST A PANEL OF PATHOGENIC BACTERIA STRAINS FORMED BY HELICOBACTER PYLORI (ATCC 43504), LISTERIA MONOCYTOGENES (ATCC 7644), STAPHYLOCOCCUS AUREUS (ATCC 9144), ESCHERICHIA COLI (ATCC 11775),AND SALMONELLA ENTERICA (ATCC 13076). PRELIMINARY FAST HPLC-MICRO FRACTIONATION ALLOWS THE IDENTIFICATION OF POTENTIAL UREASE INHIBITORS USING HIGH THROUGHPUT UREA-PHENOL MICROPLATE ASSAY. AFTERWARDS, PREPARATIVE FRACTIONATION BY CENTRIFUGAL PARTITION CHROMATOGRAPHY (CPC) ALLOW TO SELECT THE SPECIFIC BIOACTIVE FRACTIONS. A COMBINATION OF ANTIMICROBIAL TESTS, ENZYME ASSAYS AND MOLECULAR DOCKING RESULTED IN THE IDENTIFICATION BY HPLC-MS/MS OF TWO QUERCETIN-O-(6´´-O-GALLOYL)-HEXOSIDES AS THE MOST DOMINANT COMPOUNDS IN THE ACTIVE CPC-FRACTIONS. THESE BIOACTIVE COMPOUNDS WERE QUERCETIN-3-O-(6´´-O-GALLOYL)-8-GALACTOPYRANOSIDE (HYPERIN 6?-GALLATE) AND QUERCETIN-3-O-?-D-(6´´-O-GALLOYL)- ?-GLUCOPYRANOSIDE (TELLIMOSIDE). THE MOLECULAR DOCKING EVALUATION REVEALED THAT HYPERIN-6´´-GALLATE ENTER THE BINDING SITE OF UREASE AND BIND IN THROUGH PI-CATION, PI?PI, AND H-BOND INTERACTIONS. IN CONCORDANCE WITH THE IN-SILICO ASSAY, THE CPC FRACTION CONTAINING THIS COMPOUND HAS THE LOWEST VALUES OF IC50 FOR JACK BEAN (0.41 ± 0.08 ?G/ML) AND HELICOBACTER PYLORI (0.28 ± 0.08 ?G/ML) UREASES, RESPECTIVELY. MOREOVER, LABEL-FREE MICROSCALE THERMOPHORESIS (MST) ANALYSIS SUGGEST THAT THIS FLAVONOID FORMS A COMPLEX WITH UREASE, EVEN INDUCING PROTEIN AGGREGATION.