THIS RESEARCH AIMED TO EVALUATE BLOCK FREEZE CONCENTRATION (BFC) UNDER DIFFERENT CENTRIFUGATION CONDITIONS USING RESPONSE SURFACE METHODOLOGY TO SEPARATE AN EXTRACT FROM THE ICE FRACTION AT THREE CENTRIFUGAL-BFC (CBFC) CYCLES, OBTAINING IN THE FINAL CYCLE A PHENOLIC- RICH EXTRACT. A BOX?BEHNKEN DESIGN WAS APPLIED TO OPTIMIZE CENTRIFUGATION VARIABLES, WITH EFFICIENCY OF SEPARATION (?) SELECTED AS THE RESPONSE VARIABLE. THE EXTRACTS WERE CHARACTERIZED IN TERMS OF PHYSICOCHEMICAL ANALYSIS, TOTAL AND INDIVIDUAL BIOACTIVE COMPONENTS, AND ANTIOXIDANT CAPACITY. OPTIMAL CONDITIONS (3600 RPM, 16 ?C, AND 14 MIN) RESULTED IN ? OF 82%. THUS, FROM INFUSION TO FINAL CYCLE, THE SOLIDS, TOTAL POLYPHENOL AND FLAVONOID CONTENTS, AND ANTIOXIDANT CAPACITY EXHIBITED FROM 1.81 TO 6.5% (W/W) AND 2.5 TO 8.7 (?BRIX), 0.72 TO 12.2 MG GALLIC ACID EQUIVALENTS/ML, 0.83 TO 13.7 MG CATEQUIN EQUIVALENTS /ML, 2.8 TO 31.2 ?MOL TROLOX EQUIVALENTS/ML AND 4.8 TO 122.2 ?MOL TROLOX EQUIVALENTS/ML, IDENTIFYING BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY THAT KAEMPFEROL, P-HYDROXYBENZOIC, AND TRANSFERULIC ACID PRESENTED THE HIGHEST CONCENTRATIONS. THE CBFC PROCESS HAS THE POTENTIAL AS A NON-THERMAL CONCENTRATION PROCESS TO PRESERVE MANY BIOACTIVE COMPOUNDS, FACILITATING THE PRODUCTION OF CONCENTRATED FRACTIONS WITH HIGH BIOLOGICAL VALUE, WHERE THE EXTRACTS OBTAINED BY BFC ARE A NOVEL SOLUTION FOR MEDICINAL, PHARMACEUTICAL, AND FOOD APPLICATIONS.

FREEZING IS AN EFFECTIVE TECHNOLOGY WITH WHICH TO MAINTAIN FOOD QUALITY. HOWEVER, THE FORMATION OF ICE CRYSTALS DURING THIS PROCESS CAN CAUSE DAMAGE TO THE CELLULAR STRUCTURE, LEADING TO FOODDETERIORATION. A GOOD UNDERSTANDING OF THE RELATIONSHIP BETWEEN FOOD MICROSTRUCTURE AND ICE MORPHOLOGY, AS WELL AS THE ABILITY TO EFFECTIVELY MEASURE AND CONTROL ICE CRYSTALS, IS VERY USEFUL TO ACHIEVEHIGH-QUALITY FROZEN FOODS. HENCE, A BRIEF DISCUSSION IS PRESENTED ON THE FUNDAMENTALS/PRINCIPLES OF OPTICAL MICROSCOPIC TECHNIQUES (LIGHT MICROSCOPY), ELECTRONIC MICROSCOPIC TECHNIQUES (TRANSMISSION ELECTRON MICROSCOPY (TEM) AND SCANNING ELECTRON MICROSCOPY (SEM)), AS WELL AS OTHER NON-INVASIVE TECHNIQUES (X-RAYS, SPECTROSCOPY, AND MAGNETIC RESONANCE) AND THEIR APPLICATION TO MEASURING ICE FORMATION RATES AND CHARACTERIZING ICE CRYSTALS, PROVIDING INSIGHT INTO THE FREEZING MECHANISMS AS WELL AS DIRECT MONITORING OF THE ENTIRE PROCESS. AND, IN ADDITION, THIS REVIEW COMPARES (THE NEGATIVE AND POSITIVE ASPECTS OF) THE USE OF SIMPLE AND CHEAP BUT DESTRUCTIVE TECHNOLOGIES (OPTICAL MICROSCOPY) WITH DETAILED MICROSCOPIC TECHNOLOGIES AT THE MICRO/NANOMETER SCALE BUT WITH PRETREATMENTS THAT ALTER THE ORIGINAL SAMPLE (SEM AND TEM), AND NON-DESTRUCTIVE TECHNOLOGIES THAT DO NOT REQUIRE SAMPLE PREPARATION BUT WHICH HAVE HIGH ACQUISITION AND OPERATIONAL COSTS. ALSO INCLUDED ARE IMAGES AND EXAMPLES WHICH DEMONSTRATE HOW USEFUL AN ANALYSIS USING THESE TECHNIQUES CAN BE.