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A ROBUST QUANTITATIVE APPROACH FOR LASER SPECKLE CONTRAST IMAGING PERFUSION ANALYSIS REVEALED ANOMALIES IN THE BRAIN BLOOD FLOW IN OFFSPRING MICE OF PREECLAMPSIA

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dc.creatorJESENIA MARIANELA ACURIO JÁCOME
dc.creatorCARLOS ALONSO ESCUDERO OROZCO
dc.creatorPATRICIO ANDRÉS CUMSILLE ATALA
dc.date2022
dc.date.accessioned2025-01-10T15:34:36Z
dc.date.available2025-01-10T15:34:36Z
dc.date.issued2022
dc.description.abstractMICROCIRCULATION ANALYSIS OF THE BRAIN CORTEX IS CHALLENGING BECAUSE SURFACE PERFUSION VARIES RAPIDLY IN SMALL SPACE-TIME REGIONS AND IS BONE PROTECTED. THE LASER SPECKLE CONTRAST IMAGING (LSCI) TECHNIQUE ALLOWS ANALYZING IN VIVO BRAIN VASCULAR PERFUSION GENERATING A LARGE AMOUNT OF DATA THAT REQUIRES SOPHISTICATED DATA ANALYTICS, MAKING RESEARCHERS INVEST MUCH EFFORT IN PROCESSING. OUR RESEARCH QUESTION WAS WHETHER THE REDUCED PLACENTAL PERFUSION MODEL (RUPP) OF PREECLAMPSIA (PE) WAS ASSOCIATED WITH IMPAIRED BLOOD PERFUSION IN THE OFFSPRING'S BRAINS. WE AIMED TO DEVELOP A ROBUST NUMERICAL APPROACH THAT MAINLY CONSISTED OF APPLYING A SIGNAL-PROCESSING TOOL FOR CALCULATING OPTIMAL SEGMENTATION AND PIECE-WISE FITS OF THE OFFSPRING'S BRAIN PERFUSION SIGNALS OBTAINED FROM THE LSCI TECHNIQUE. WE COMBINED THIS TOOL WITH THE USUAL STATISTICAL ANALYSIS, IMPLEMENTING BOTH IN MATLAB SOFTWARE. WE PERFORMED BRAIN PERFUSION MEASUREMENTS FROM OFFSPRING (FIVE DAYS POSTNATAL, P5) OF CONTROL PREGNANT DAMS (SHAM, N = 13) AND OF RUPP DAMS (RUPP, N = 7) USING THE PERICAM® PSI-HR SYSTEM AT A BASAL CONDITION AND AFTER THERMAL STIMULI (WARM AND COLD). WE FOUND THAT PUPS OF RUPP MICE EXHIBITED SIGNIFICANT DIFFERENCES IN PERFUSION AND VASCULAR RESPONSE TO THERMAL STIMULI COMPARED TO THE SHAM MICE. THESE DIFFERENCES WERE ASSOCIATED WITH HIGH DATA VARIABILITY IN THE SHAM GROUP, WHILE IN THE RUPP GROUP, PERFUSION LOOKS ?STIFFER.? DATA ALSO SUGGEST SEXDIMORPHISM IN THE VASCULAR RESPONSE SINCE FEMALE PUPS IN THE SHAM GROUP BUT NOT MALE PUPS SHOWED STATISTICALLY SIGNIFICANT DIFFERENCES IN RESPONSE TO THE WARM STIMULUS. AGAIN, THIS SEX-RELATED DIFFERENCE WAS ABSENT IN PUPS OF RUPP MICE. IN CONCLUSION, WE PRESENT A ROBUST QUANTITATIVE APPROACH FOR LSCI MEASUREMENTS THAT REVEALED ANOMALIES IN THE BRAIN BLOOD FLOW IN OFFSPRING OF THE RUPP MODEL OF PE.
dc.formatapplication/pdf
dc.identifier.doi10.1016/j.mvr.2022.104418
dc.identifier.issn1095-9319
dc.identifier.issn0026-2862
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/12666
dc.languagespa
dc.publisherMICROVASCULAR RESEARCH
dc.relation.uri10.1016/j.mvr.2022.104418
dc.rightsPUBLICADA
dc.subjectVascular response
dc.subjectSex-dimorphism
dc.subjectRUPP model of preeclampsia
dc.subjectQuantitative approach
dc.subjectLaser speckle contrast imaging
dc.subjectCognitive decline
dc.subjectBrain microcirculation
dc.subjectRESPUESTA VASCULAR
dc.subjectMODELO RUPP DE PREECLAMPSIA
dc.subjectMICROCIRCULACIÓN CEREBRAL
dc.subjectIMÁGENES DE CONTRASTE MOTEADO LÁSER
dc.subjectENFOQUE CUANTITATIVO
dc.subjectDIMORFISMO SEXUAL
dc.subjectDETERIORO COGNITIVO
dc.titleA ROBUST QUANTITATIVE APPROACH FOR LASER SPECKLE CONTRAST IMAGING PERFUSION ANALYSIS REVEALED ANOMALIES IN THE BRAIN BLOOD FLOW IN OFFSPRING MICE OF PREECLAMPSIA
dc.title.alternativeUN ENFOQUE CUANTITATIVO ROBUSTO PARA EL ANÁLISIS DE PERFUSIÓN DE IMÁGENES DE CONTRASTE DE MOTEADO LÁSER REVELÓ ANOMALÍAS EN EL FLUJO SANGUÍNEO CEREBRAL EN RATONES DESCENDIENTES DE PREECLAMPSIA
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS DE REHABILITACION EN SALUD
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
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