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EFFECT OF IN VITRO DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOUS OF LEAVES OF UGNI MOLINAE, ON THE VIABILITY OF COLORECTAL CANCER CELLS

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dc.creatorROMINA IVONNE CARVAJAL CARVAJAL
dc.creatorEILLEN ROMANET TORRES VALLEJOS
dc.creatorEDGAR RAFAEL PASTENE NAVARRETE
dc.date2021
dc.date.accessioned2025-01-10T15:22:52Z
dc.date.available2025-01-10T15:22:52Z
dc.date.issued2021
dc.description.abstractTHIS WORK IS AIMED TO THE STUDY THE EFFECT OF AQUEOUS EXTRACTS OBTAINED FROM LEAVES OF UGNI MOLINAE AND THEIR RESPECTIVE PRODUCTS OF IN VITRO GASTROINTESTINAL DIGESTION ON THE VIABILITY OF COLON CANCER CELLS. METHODS: UGNI MOLINAE LEAVES WERE USED TO PREPARE A 1%, AQUEOUS EXTRACT AND WITH THIS, AN IN VITRO GASTROINTESTINAL DIGESTION WAS PERFORMED, OBTAINING, FRACTION PRODUCTS OF DIGESTION GASTROINTESTINAL (DGI) AND FRACTION RESIDUE. THIS FRACTION WAS IDENTIFIED THROUGH LIQUID CHROMATOGRAPHY (HPLC-UV) AND LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (HPLC-MS), AND THEN THESE SAMPLES WERE EVALUATED IN DIFFERENT VIABILITY TESTS, SUCH AS TRYPAN BLUE EXCLUSION, METABOLIC ACTIVITY 3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) AND CYTOTOXICITY BY LACTATE DEHYDROGENASE (LDH), ON COLORECTAL CANCER CELLS (CACO-2) AND HUMAN EMBRYONIC KIDNEY 293 CELLS (HEK) FOR A PERIOD OF 24 AND 48 HOURS. RESULTS AND DISCUSSION: THE AQUEOUS EXTRACTS OF LEAVES OF UGNI MOLINAE AND THEIR DIGESTION FRACTIONS CAUSED INHIBITION OF THE VIABILITY OF CACO-2 CELLS. IN MOST CASES, THE EFFECT WAS CONCENTRATION-DEPENDENT AND IN PARTICULAR, THE ONLY COMPOUND THAT GENERATED INHIBITION OF THE VIABILITY IN ALL TRIALS CORRESPONDED TO THE DGI FRACTION. CONCLUSION: THE RESULTS ARE FAVORABLE, SINCE IT IS FINALLY IS THIS PRODUCT THAT WOULD ACT IN THE GASTROINTESTINAL TRACT, INTERACTING WITH THE TARGET CELLS. THESE RESULTS ARE IMPORTANT SINCE IN MOST CASES, THESE COMPOUNDS HAD EFFECTS ON CANCER CELLS CACO-2 AND NOT ON HEK CELLS AT THE SAME CONCENTRATIONS USED IN EACH TRIAL.
dc.formatapplication/pdf
dc.identifier.doi10.4067/S0717-97072021000305268
dc.identifier.issn0717-9707
dc.identifier.issn0717-9707
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/11742
dc.languagespa
dc.publisherJOURNAL OF THE CHILEAN CHEMICAL SOCIETY
dc.relation.uri10.4067/S0717-97072021000305268
dc.rightsPUBLICADA
dc.titleEFFECT OF IN VITRO DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOUS OF LEAVES OF UGNI MOLINAE, ON THE VIABILITY OF COLORECTAL CANCER CELLS
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionPROGRAMA FERIA DE LA CIENCIA
ubb.Otra ReparticionDIRECCION DE INVESTIGACION Y CREACION ARTISTICA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCONCEPCIÓN
ubb.SedeCONCEPCIÓN
ubb.SedeCHILLÁN
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