Publicación:
ELEVATED PRO-ANGIOGENIC PHENOTYPE IN FETO-PLACENTAL TISSUE FROM GESTATIONAL DIABETES MELLITUS

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dc.creatorJESENIA MARIANELA ACURIO JÁCOME
dc.creatorKURT ERWIN HERLITZ FLORES
dc.creatorCARLOS ALONSO ESCUDERO OROZCO
dc.date2015
dc.date.accessioned2025-01-10T14:38:00Z
dc.date.available2025-01-10T14:38:00Z
dc.date.issued2015
dc.description.abstractOBJECTIVE: WE AIMED TO INVESTIGATE WHETHER FETO-PLACENTAL TISSUE FROM GESTATIONAL DIABETES MELLITUS (GDM) EXHIBITS AN ELEVATED PRO-ANGIOGENIC PHENOTYPE COMPARED TO NORMAL PREGNANCY. METHODS: WE OBTAINED PLACENTAL SAMPLES FROM NORMAL PREGNANT WOMEN (N=12) AND GDM PATIENTS (N=14), WHICH WERE HOMOGENIZED TO OBTAIN MRNA AND PROTEIN EXTRACTIONS. SEMI-QUANTITATIVE PCR WAS PERFORMED TO ESTIMATE MRNA LEVELS OF VEGF, VEGFR TYPE 1 (VEGFR1) AND VEGFR2. ALSO, PROTEIN CONCENTRATIONS OF CD31, CD34, KI67, VEGF, VEGFR1, VEGFR2 AND Y951-PHOSPHORYLATED VEGFR2 WERE ESTIMATED BY WESTERN BLOT. Y1175 PHOSPHORYLATED VEGFR2 WAS MEASURED BY ELISA. ALSO HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVEC) WERE ISOLATED FROM NORMAL (N=10) AND GDM (N=10) WOMAN. CELL PROLIFERATION WAS ANALYZED BY COLORIMETRIC MTS-ASSAY (PROMEGA, USA), WHEREAS MIGRATION WAS ANALYZED BY CELL-SCRATCH ASSAY AFTER 24 HOURS OF CELL INCUBATION WITHOUT ANY TREATMENT.
dc.formatapplication/pdf
dc.identifier.doi10.1016/j.placenta.2015.01.436
dc.identifier.issn1532-3102
dc.identifier.issn0143-4004
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/8282
dc.languagespa
dc.publisherPLACENTA
dc.relation.uri10.1016/j.placenta.2015.01.436
dc.rightsPUBLICADA
dc.titleELEVATED PRO-ANGIOGENIC PHENOTYPE IN FETO-PLACENTAL TISSUE FROM GESTATIONAL DIABETES MELLITUS
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
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