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ADAPTATION OF THE TWEEN 80 ASSAY WITH A RESOLUTION V FRACTIONAL FACTORIAL DESIGN AND ITS APPLICATION TO RANK OPHIOSTOMA FUNGI WITH WOOD EXTRACTIVE DEGRADING CAPABILITIES

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dc.creatorPAULA MARCELA HERRERA HERRERA
dc.creatorENRIQUE ADALBERTO WERNER NAVARRETE
dc.creatorJOSÉ ARTURO NAVARRETE ARAYA
dc.date2015
dc.date.accessioned2025-01-10T14:30:34Z
dc.date.available2025-01-10T14:30:34Z
dc.date.issued2015
dc.description.abstractWOOD EXTRACTIVES IN RADIATA PINE ARE THE SOURCE OF THE OBSERVED PITCH PROBLEMS IN THE PULP AND PAPER INDUSTRY. VARIOUS METHODOLOGIES HAVE BEEN STUDIED AND USED TO REDUCE OR ELIMINATE THEIR NEGATIVE EFFECTS. A BIOLOGICAL TREATMENT WITH ALBINO FUNGI OF THE OPHIOSTOMA GENUS, FUNGI THAT DEGRADE THE LIPIDIC COMPONENTS OF EXTRACTIVES, HAS BEEN PROPOSED AS A MORE ENVIRONMENTALLY FRIENDLY ALTERNATIVE FOR PITCH DEGRADATION. THE CURRENT METHODS USED TO SEARCH FOR OPHIOSTOMA ALBINO STRAINS WITH THE HIGHEST DEGRADATION RATES OF WOOD EXTRACTIVES ARE LABOR INTENSIVE AND REQUIRE A LARGE AMOUNT OF RESOURCES. THE TWEEN 80® OPACITY TEST, AN ASSAY DESIGNED TO MEASURE LIPOLYTIC ENZYME ACTIVITY IN FILAMENTOUS FUNGI, WAS STUDIED TO VERIFY THE FEASIBILITY OF ITS APPLICATION AS A METHODOLOGY TO RANK OPHIOSTOMA ALBINO STRAINS WITH DERESINATION CAPABILITIES. THE TWEEN 80® OPACITY TEST WAS FIRST CHARACTERIZED BY IMPLEMENTING A NON-REPLICATED 2K-P FRACTIONAL FACTORIAL DESIGN OF RESOLUTION V FOR A 5 FACTORS WITH 16 TREATMENTS AND THEN TO STUDY THE EFFECT OF THE FUNGUS SPECIES ON THE LIPOLYTIC ENZYME ACTIVITY; A RANDOMIZED ONE FACTOR GENERAL FACTORIAL DESIGN WAS CONDUCTED. THE INCUBATION TEMPERATURE; ANTIBIOTICS PRESENCE; AND TWEEN 80®, CACL2 , AND PEPTONE CONCENTRATIONS WERE INVESTIGATED IN THE FIRST EXPERIMENT. THE OPHIOSTOMA SPECIES EFFECT WAS STUDIED IN THE SECOND EXPERIMENT. IN BOTH EXPERIMENTS, THE HALO AREA SIZE, WHICH WAS FORMED BY THE FATTY ACID-CALCIUM COMPLEX PRECIPITATE, WAS THE RESPONSE VARIABLE. THE RESULTS DEMONSTRATED THE EFFECTIVENESS OF THE TWEEN 80® OPACITY TEST TO MEASURE THE LIPOLYTIC ENZYME ACTIVITY OF OPHIOSTOMA ALBINO STRAINS. SIMILARLY, THE INCUBATION TEMPERATURE AND THE CONCENTRATIONS OF TWEEN 80®, CACL2 , AND BACTERIOLOGICAL PEPTONE HAD THE HIGHEST STATISTICALLY SIGNIFICANT EFFECT ON THE RESPONSE VARIABLE. IN ADDITION, OUR DATA DEMONSTRATED THAT THE ALBINO STRAINS FROM THE SPECIE O. FLOCCOSUM EXHIBITED THE HIGHEST RATE OF LIPOLYTIC ENZYME PRODUCTION.
dc.formatapplication/pdf
dc.identifier.doi10.4067/S0718-221X2015005000009
dc.identifier.issn0717-3644
dc.identifier.issn0718-221X
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/7742
dc.languagespa
dc.publisherMADERAS: CIENCIA Y TECNOLOGIA
dc.relation.uri10.4067/S0718-221X2015005000009
dc.rightsPUBLICADA
dc.subjectPITCH CONTROL
dc.subjectOPHIOSTOMA PILIFERUM
dc.subjectOPHIOSTOMA PICEAE
dc.subjectOPHIOSTOMA FLOCCOSUM
dc.subjectLIPASE ENZYME ACTIVITY
dc.subjectFACTORIAL DESIGN
dc.titleADAPTATION OF THE TWEEN 80 ASSAY WITH A RESOLUTION V FRACTIONAL FACTORIAL DESIGN AND ITS APPLICATION TO RANK OPHIOSTOMA FUNGI WITH WOOD EXTRACTIVE DEGRADING CAPABILITIES
dc.title.alternativeADAPTACIÓN DEL ENSAYO TWEEN 80 CON UN DISEÑO FACTORIAL FRACCIONAL DE RESOLUCIÓN V Y SU APLICACIÓN PARA CLASIFICAR HONGOS OFIOSTOMA CON CAPACIDADES DE DEGRADACIÓN EXTRACTIVA DE MADERA
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionPROGRAMA DE BIODETERIORO
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.Otra ReparticionDEPARTAMENTO DE INGENIERIA EN MADERAS
ubb.SedeCONCEPCIÓN
ubb.SedeCHILLÁN
ubb.SedeCONCEPCIÓN
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