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CHARACTERIZATION OF CRONOBACTER SAKAZAKII AND CRONOBACTER MALONATICUS STRAINS ISOLATED FROM POWDERED DAIRY PRODUCTS INTENDED FOR CONSUMPTION BY ADULTS AND OLDER ADULTS

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dc.creatorJULIO ENRIQUE PARRA FLORES
dc.date2023
dc.date.accessioned2025-01-10T15:43:36Z
dc.date.available2025-01-10T15:43:36Z
dc.date.issued2023
dc.description.abstractTHE OBJECTIVE OF THIS STUDY WAS TO CHARACTERIZE CRONOBACTER SPP. AND RELATED ORGANISMS ISOLATED FROM POWDER DAIRY PRODUCTS INTENDED FOR CONSUMPTION BY ADULTS AND OLDER ADULTS USING WHOLE-GENOME SEQUENCING (WGS), AND TO IDENTIFY GENES AND TRAITS THAT ENCODE ANTIBIOTIC RESISTANCE AND VIRULENCE. VIRULENCE (VGS) AND ANTIBIOTIC RESISTANCE GENES (ARGS) WERE DETECTED WITH THE COMPREHENSIVE ANTIBIOTIC RESISTANCE DATABASE (CARD) PLATFORM, RESFINDER, AND MOB-SUITE TOOLS. SUSCEPTIBILITY TESTING WAS PERFORMED USING DISK DIFFUSION. FIVE PRESUMPTIVE STRAINS OF CRONOBACTER SPP. WERE IDENTIFIED BY MALDI?TOF MS AND RIBOSOMAL MLST. THREE C. SAKAZAKII STRAINS WERE OF THE CLINICAL PATHOVAR ST1, ONE WAS ST31, AND THE REMAINING ISOLATE WAS C. MALONATICUS ST60. IN ADDITION, FRANCONIBACTER HELVETICUS ST345 WAS IDENTIFIED. THE C. SAKAZAKII ST1 STRAINS WERE FURTHER DISTINGUISHED USING CORE GENOME MLST BASED ON 2831 LOCI. MOREOVER, 100% OF THE STRAINS WERE RESISTANT TO CEFALOTIN, 75% TO AMPICILLIN, AND 50% TO AMIKACIN. THE C. SAKAZAKII ST1 STRAINS WERE MULTIRESISTANT (MDR) TO FOUR ANTIBIOTICS. ADDITIONALLY, ALL THE STRAINS ADHERED TO THE N1E-115 CELL LINE, AND TWO INVADED IT. EIGHTEEN ARGS MAINLY INVOLVED IN ANTIBIOTIC TARGET ALTERATION AND ANTIBIOTIC EFFLUX WERE DETECTED. THIRTY VGS WERE DETECTED AND CLUSTERED AS FLAGELLAR PROTEINS, OUTER MEMBRANE PROTEINS, CHEMOTAXIS, HEMOLYSINS, AND GENES INVOLVED IN METABOLISM AND STRESS. THE PESA3, PSP291-1, AND PCMA1 PLASMIDS WERE DETECTED, AND THE PREVALENT MOBILE GENETIC ELEMENTS (MGES) WERE ISESA1, ISEC52, AND IS26. THE ISOLATES OF C. SAKAZAKII AND C. MALONATICUS EXHIBITED MULTIRESISTANCE TO ANTIBIOTICS, HARBORED GENES ENCODING VARIOUS ANTIBIOTIC RESISTANCE PROTEINS, AND VARIOUS VIRULENCE FACTORS. CONSEQUENTLY, THESE CONTAMINATED POWDERED DAIRY PRODUCTS POSE A RISK TO THE HEALTH OF HYPERSENSITIVE ADULTS.
dc.formatapplication/pdf
dc.identifier.doi10.3390/microorganisms11122841
dc.identifier.issn2076-2607
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/13371
dc.languagespa
dc.publisherMICROORGANISMS
dc.relation.uri10.3390/microorganisms11122841
dc.rightsPUBLICADA
dc.subjectwhole-genome sequencing
dc.subjectvirulence factors
dc.subjectpowder dairy products
dc.subjectolder adults
dc.subjectMDR
dc.subjectCronobacter sakazakii
dc.subjectCronobacter malonaticus
dc.titleCHARACTERIZATION OF CRONOBACTER SAKAZAKII AND CRONOBACTER MALONATICUS STRAINS ISOLATED FROM POWDERED DAIRY PRODUCTS INTENDED FOR CONSUMPTION BY ADULTS AND OLDER ADULTS
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE NUTRICION Y SALUD PUBLICA
ubb.SedeCHILLÁN
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