Publicación: GABAPENTIN INHIBITS MULTIPLE STEPS IN THE AMYLOID BETA TOXICITY CASCADE
Fecha
2020
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ACS CHEMICAL NEUROSCIENCE
Resumen
OLIGOMERIC ?-AMYLOID PEPTIDE (A?) IS ONE OF THE MAIN NEUROTOXIC AGENTS OF ALZHEIMERS DISEASE (AD). OLIGOMERS ASSOCIATE TO NEURONAL MEMBRANES, FORMING """"PORE-LIKE"""" STRUCTURES THAT CAUSE INTRACELLULAR CALCIUM AND NEUROTRANSMITTER DYSHOMEOSTASIS, LEADING TO SYNAPTIC FAILURE AND DEATH. THROUGH MOLECULAR SCREENING TARGETING THE C TERMINAL REGION OF A?, A REGION INVOLVED IN THE TOXIC PROPERTIES OF THE PEPTIDE, WE DETECTED AN FDA APPROVED COMPOUND, GABAPENTIN (GBP), WITH NEUROPROTECTIVE EFFECTS AGAINST A? TOXICITY. AT MICROMOLAR CONCENTRATIONS, GBP ANTAGONIZED PEPTIDE AGGREGATION OVER TIME AND REDUCED THE A? ABSORBANCE PLATEAU TO 28% OF CONTROL. IN ADDITION, GBP DECREASED A? ASSOCIATION TO MEMBRANES BY ALMOST HALF, AND THE EFFECTS OF A? ON INTRACELLULAR CALCIUM IN HIPPOCAMPAL NEURONS WERE ANTAGONIZED WITHOUT CAUSING EFFECTS ON ITS OWN. FINALLY, WE FOUND THAT GBP WAS ABLE TO BLOCK THE SYNAPTOTOXICITY INDUCED BY A? IN HIPPOCAMPAL NEURONS, INCREASING POST-SYNAPTIC CURRENTS FROM 1.7 ± 0.9 TO 4.2 ± 0.7 FC AND MEAN RELATIVE FLUORESCENCE INTENSITY VALUES OF SV2, A SYNAPTIC PROTEIN, FROM 0.7 ± 0.09 TO 1.00 ± 0.08. THE RESULTS SHOW THAT GBP CAN INTERFERE WITH A?-INDUCED TOXICITY BY BLOCKING MULTIPLE STEPS, RESULTING IN NEUROPROTECTION, WHICH JUSTIFIES ADVANCING TOWARD ADDITIONAL ANIMAL AND HUMAN STUDIES.
