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PREECLAMPSIA AND INCREASED PERMEABILITY OVER THE BLOOD-BRAIN BARRIER: A ROLE OF VASCULAR ENDOTHELIAL GROWTH RECEPTOR 2

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dc.creatorJESENIA MARIANELA ACURIO JÁCOME
dc.creatorEVELYN ELIA LARA NAVARRETE
dc.creatorCARLOS ALONSO ESCUDERO OROZCO
dc.date2021
dc.date.accessioned2025-01-10T15:26:17Z
dc.date.available2025-01-10T15:26:17Z
dc.date.issued2021
dc.description.abstractBACKGROUND: CEREBRAL COMPLICATIONS IN PREECLAMPSIA ARE LEADING CAUSES OF MATERNAL MORTALITY WORLDWIDE BUT PATHOPHYSIOLOGY IS LARGELY UNKNOWN AND A CHALLENGE TO STUDY. USING AN IN VITRO MODEL OF THE HUMAN BLOOD-BRAIN BARRIER (BBB), WE EXPLORED THE ROLE OF VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2 (VEGFR2) IN PREECLAMPSIA. METHODS: THE HUMAN BRAIN ENDOTHELIAL CELL LINE (HCMEC/D3) CULTURED ON TRANWELLS INSERT WAS EXPOSED (12 HOURS) TO PLASMA FROM WOMEN WITH PREECLAMPSIA (N = 28), NORMAL PREGNANCY (N = 28), AND NONPREGNANT (N = 16) CONTROLS. TRANSENDOTHELIAL ELECTRICAL RESISTANCE (TEER) AND PERMEABILITY TO 70 KDA FLUORESCEIN ISOTHIOCYANATE (FITC)-DEXTRAN WERE MEASURED FOR THE ASSESSMENT OF BBB INTEGRITY. WE EXPLORED POSSIBLE UNDERLYING MECHANISMS, WITH A FOCUS ON THE EXPRESSION OF TIGHT JUNCTION PROTEINS AND PHOSPHORYLATION OF 2 TYROSINE RESIDUES OF VEGFR2, ASSOCIATED WITH VASCULAR PERMEABILITY AND MIGRATION (PY951) AND CELL PROLIFERATION (PY1175). PLASMA CONCENTRATIONS OF SOLUBLE FMS-LIKE TYROSINE KINASE-1 (SFLT-1) AND PLACENTAL GROWTH FACTOR (PLGF) WERE ALSO MEASURED. RESULTS: HCMEC/D3 EXPOSED TO PLASMA FROM WOMEN WITH PREECLAMPSIA EXHIBITED REDUCED TEER AND INCREASED PERMEABILITY TO 70 KDA FITC-DEXTRAN. THESE CELLS UPREGULATED THE MESSENGER RIBONUCLEIC ACID (MRNA) LEVELS OF VEGFR2, AND PY951-VEGFR2, BUT REDUCED PY1175-VEGFR2 (P < 0.05 IN ALL CASES). NO DIFFERENCE IN MRNA EXPRESSION OF TIGHT JUNCTION PROTEIN WAS OBSERVED BETWEEN GROUPS. THERE WAS NO CORRELATION BETWEEN ANGIOGENIC BIOMARKERS AND BBB PERMEABILITY. CONCLUSIONS: WE PRESENT A PROMISING IN VITRO MODEL OF THE BBB IN PREECLAMPSIA. SELECTIVE TYROSINE PHOSPHORYLATION OF VEGFR2 MAY PARTICIPATE IN THE INCREASED BBB PERMEABILITY IN PREECLAMPSIA IRRESPECTIVE OF PLASMA CONCENTRATIONS OF ANGIOGENIC BIOMARKERS.
dc.formatapplication/pdf
dc.identifier.doi10.1093/ajh/hpaa142
dc.identifier.issn1941-7225
dc.identifier.issn0895-7061
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/12013
dc.languagespa
dc.publisherAMERICAN JOURNAL OF HYPERTENSION
dc.relation.uri10.1093/ajh/hpaa142
dc.rightsPUBLICADA
dc.titlePREECLAMPSIA AND INCREASED PERMEABILITY OVER THE BLOOD-BRAIN BARRIER: A ROLE OF VASCULAR ENDOTHELIAL GROWTH RECEPTOR 2
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS DE REHABILITACION EN SALUD
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
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