Publicación: IS ANGIOGENESIS ALTERED IN THE OFFSPRING OF MOTHERS WITH PREECLAMPSIA? POTENTIAL ROLE OF A2A ADENOSINE RECEPTOR-NITRIC OXIDE-VASCULAR ENDOTHELIAL GROWTH FACTOR PATHWAY
Fecha
2017
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PLACENTA
Resumen
INDIRECT EVIDENCES SUGGEST REDUCED ANGIOGENESIS IN OFFSPRING BORN TO PREECLAMPSIA. ADENOSINE, VIA NITRIC OXIDE (NO) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) MAY REGULATE ANGIOGENESIS.
OBJECTIVES: TO INVESTIGATE WHETHER A2A ADENOSINE RECEPTOR (A2AAR)/NO/VEGF EXPRESSION IS IMPAIRED IN OFFSPRING FROM PRE-ECLAMPSIA.
METHODS: IN HUMAN, CULTURES OF UMBILICAL VEIN ENDOTHELIAL CELLS (HUVEC) AND PLACENTAL MICROVASCULAR ENDOTHELIAL CELLS (HPMEC) FROM NORMAL PREGNANCIES AND WOMEN WITH PREECLAMPSIA WERE USED FOR ESTIMATION OF MRNA LEVELS OF VEGF, CELL PROLIFERATION AND ANGIOGENESIS IN VITRO IN PRESENCE OR ABSENCE OF SIRNA FOR A2AAR. ALSO, PREECLAMPSIA-LIKE SYNDROME WAS GENERATED IN PREGNANT MICE (C56BJ) EXPOSED TO THE NO SYNTHASE INHIBITOR, NG-NITRO-L-ARGININE METHYL ESTER (L-NAME, 50 MG/KG). IN VIVO BLOOD PERFUSION USING LASER DOPPLER WAS ANALYSED AT DAY 7 POSTNATAL IN OFFSPRING (F1), EXPOSED OR NOT TO PREECLAMPSIA-LIKE SYNDROME.
