Publicación:
AQUEOUS EXTRACT OF PULP OF MAQUI-BERRY (ARISTOTELIA CHILENSIS) INDUCES APOPTOSIS IN HUMAN ENDOMETRIAL CARCINOMA ISHIKAWA CELLS VIA MITOCHONDRIAL AND ENDOPLASMIC RETICULUM STRESS PATHWAYS

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dc.creatorCARLA ESTEFANY VIDAL SAN MARTÍN
dc.creatorCARLOS LEONARDO ARMANDO CÉSPEDES ACUÑA
dc.creatorJULIO ENRIQUE ILDEFONSO ALARCÓN ENOS
dc.creatorJOSÉ MIGUEL BASTÍAS MONTES
dc.date2024
dc.date.accessioned2025-01-10T15:52:52Z
dc.date.available2025-01-10T15:52:52Z
dc.date.issued2024
dc.description.abstractIN CHILEAN TRADITIONAL MEDICINE, ARISTOTELIA CHILENSIS (MOL) STUNTZ IS COMMONLY UTILIZED FOR A VARIETY OF CONDITIONS (PARTICULARLY DIGESTIVE PROBLEMS). WE INVESTIGATED THE EFFECTS OF AN AQUEOUS EXTRACT FROM THE FRUIT PULP OF A. CHILENSIS (MAQUI BERRY: M2) ON ENDOPLASMIC RETICULUM STRESS AND MITOCHONDRIAL PATHWAY-INDUCED EARLY APOPTOSIS IN ISHIKAWA CELLS. USING THE MTT ASSAY, THE AQUEOUS EXTRACT WAS FOUND TO HAVE AN INHIBITORY IMPACT ON HUMAN ENDOMETRIAL CANCER ISHIKAWA CELLS, WITH AN IC50 OF 5.36 MU G/ML. THE CHANGES IN ISHIKAWA CELLS UNDER THE EFFECT OF M2 WERE DETECTED BY FLOW CYTOMETRY WITH CYCLE ARREST IN S PHASE, REDUCED MITOCHONDRIAL MEMBRANE POTENTIAL, ROS AND CALCIUM ION AGGREGATION. WESTERN BLOTTING AND RT-QPCR WERE USED TO IDENTIFY THE INTRACELLULAR MRNA AND PROTEIN ALTERATIONS. THE PRESENCE OF M2 RESULTED IN THE UP-REGULATION OF MITOCHONDRIAL PATHWAYRELATED PROTEINS BAD, BAX, AND CYTO C, DOWN-REGULATION OF BCL-2, AND UP-REGULATION OF ENDOPLASMIC RETICULUM STRESS-RELATED PROTEINS IRE1 ALPHA, P-EIF2 ALPHA, ATF4, AND CHOP. THE FINDINGS IMPLY THAT A. CHILENSIS PULP MAY BE USED AS A POSSIBLE RAW MATERIAL FOR THE INNOVATIVE FUNCTIONAL APPLICATIONS.
dc.formatapplication/pdf
dc.identifier.doi10.1016/j.fbio.2024.104733
dc.identifier.issn2212-4306
dc.identifier.issn2212-4292
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/14087
dc.languagespa
dc.publisherFOOD BIOSCIENCE
dc.relation.uri10.1016/j.fbio.2024.104733
dc.rightsPUBLICADA
dc.subjectMitochondrial pathway
dc.subjectMaqui berry
dc.subjectM2
dc.subjectEndoplasmic reticulum stress
dc.subjectCell cycle
dc.subjectAristotelia chilensis
dc.subjectApoptosis
dc.titleAQUEOUS EXTRACT OF PULP OF MAQUI-BERRY (ARISTOTELIA CHILENSIS) INDUCES APOPTOSIS IN HUMAN ENDOMETRIAL CARCINOMA ISHIKAWA CELLS VIA MITOCHONDRIAL AND ENDOPLASMIC RETICULUM STRESS PATHWAYS
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE INGENIERIA EN ALIMENTOS
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.Otra ReparticionDEPARTAMENTO DE INGENIERIA EN ALIMENTOS
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
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