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EXPRESSION OF GENES ASSOCIATED WITH INFLAMMATION AND IRON METABOLISM IN 3T3-L1 CELLS INDUCED WITH MACROPHAGES- CONDTIONED MEDIUM, GLUCOSE AND IRON

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dc.creatorLAUTARO RIGOBERTO NABI BRIONES SUÁREZ
dc.date2018
dc.date.accessioned2025-01-10T14:58:30Z
dc.date.available2025-01-10T14:58:30Z
dc.date.issued2018
dc.description.abstractOBESITY IS CHARACTERIZED BY A CHRONIC INFLAMMATORY PROCESS, WITH AN INCREASED VOLUME OF TOTAL ADIPOSE TISSUE, ESPECIALLY VISCERAL, WHICH SECRETES PRO-INFLAMMATORY CYTOKINES SUCH AS TNF-? AND IL-6. HEPCIDIN (HPC), A MAIN IRON METABOLISM REGULATOR, IS SYNTHETIZED BY AN IL-6 STIMULI, AMONG OTHERS, IN LIVER AND ADIPOSE TISSUE, FAVORING AN ASSOCIATION BETWEEN THE INFLAMMATORY PROCESS AND IRON METABOLISM. STILL THERE ARE QUESTIONS REMAIN REGARDING THE INTERACTION OF THESE FACTORS. OUR AIM WAS TO STUDY THE EFFECT OF A MACROPHAGE-CONDITIONED MEDIUM (MCM) ON ADIPOCYTE CELLS CHALLENGED WITH GLUCOSE AND/OR IRON. WE STUDIED THE MRNA RELATIVE ABUNDANCE OF GENES RELATED TO INFLAMMATION IN DIFFERENTIATED 3T3-L1 CELLS CHALLENGED WITH FE (40 ?M), GLUCOSE (20 MM) OR FE/GLUCOSE (40 ?M/20 MM) WITH OR WITHOUT MCM FOR 24 H. WE ALSO MEASURED THE INTRACELLULAR IRON LEVELS UNDER THESE CONDITIONS. OUR RESULTS SHOWED THAT WHEN ADIPOCYTES WERE CHALLENGED WITH MCM, GLUCOSE AND/OR FE, THE INTRACELLULAR IRON AND MRNA LEVELS OF PRO-INFLAMMATORY CYTOKINES INCREASED. THESE RESPONSES WERE HIGHER WHEN ALL THE STIMULI WERE COMBINED WITH MCM FROM MACROPHAGES. THUS, WE SHOWED THAT COMBINED HIGH GLUCOSE/HIGH FE ALONE OR WITH MCM MAY CONTRIBUTE TO AN INCREASE ON INTRACELLULAR IRON AND INFLAMMATORY RESPONSE IN 3T3-L1 DIFFERENTIATED CELLS, BY INCREASED MRNA LEVELS OF IL-6, TNF-?, MCP-1, HPC AND REDUCING ADIPONECTIN LEVELS, ENHANCING THE INFLAMMATORY PROCESSES.
dc.formatapplication/pdf
dc.identifier.doidoi.org/10.1007/s10534-018-0108-4
dc.identifier.issn1572-8773
dc.identifier.issn0966-0844
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/9818
dc.languagespa
dc.publisherBIOMETALS
dc.relation.uridoi.org/10.1007/s10534-018-0108-4
dc.rightsPUBLICADA
dc.titleEXPRESSION OF GENES ASSOCIATED WITH INFLAMMATION AND IRON METABOLISM IN 3T3-L1 CELLS INDUCED WITH MACROPHAGES- CONDTIONED MEDIUM, GLUCOSE AND IRON
dc.title.alternativeEXPRESIÓN DE GENES ASOCIADOS CON INFLAMACIÓN Y METABOLISMO DEL HIERRO EN CÉLULAS 3T3-L1 INDUCIDAS CON MEDIO CONDICIONADO POR MACRÓFAGOS, GLUCOSA Y HIERRO
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE NUTRICION Y SALUD PUBLICA
ubb.SedeCHILLÁN
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