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DISRUPTION OF THE BLOOD-BRAIN BARRIER BY EXTRACELLULAR VESICLES FROM PREECLAMPSIA PLASMA AND HYPOXIC PLACENTAE: ATTENUATION BY MAGNESIUM SULFATE

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dc.creatorJESENIA MARIANELA ACURIO JÁCOME
dc.creatorCARLOS ALONSO ESCUDERO OROZCO
dc.date2021
dc.date.accessioned2025-01-10T15:23:32Z
dc.date.available2025-01-10T15:23:32Z
dc.date.issued2021
dc.description.abstractPREECLAMPSIA, A PREGNANCY-RELATED ENDOTHELIAL DISORDER, IS ASSOCIATED WITH BOTH CARDIOVASCULAR AND CEREBROVASCULAR COMPLICATIONS. PREECLAMPSIA REQUIRES THE PRESENCE OF A PLACENTA AS PART OF ITS PATHOPHYSIOLOGY, YET THE ROLE OF THIS ORGAN IN THE CEREBROVASCULAR COMPLICATIONS REMAINS UNCLEAR. RESEARCH HAS SHOWN THAT CIRCULATING SMALL EXTRACELLULAR VESICLES (ALSO KNOWN AS EXOSOMES) PRESENT IN PREECLAMPSIA PLASMA CAN GENERATE ENDOTHELIAL DYSFUNCTION, BUT IT IS UNCLEAR WHETHER THE IMPAIRMENT OF FUNCTION OF BRAIN ENDOTHELIAL CELLS AT THE BLOOD-BRAIN BARRIER IS SECONDARY TO PLASMA-DERIVED OR PLACENTAL-DERIVED EXOSOMES. IN THIS STUDY, WE EVALUATED THE EFFECT OF SMALL EXTRACELLULAR VESICLES ISOLATED FROM PLASMA SAMPLES OF WOMEN WITH PREECLAMPSIA (N=12) AND WOMEN WITH NORMAL PREGNANCY (N=11) AS WELL AS FROM HUMAN PLACENTAL EXPLANTS FROM NORMOTENSIVE PREGNANCIES (N=6) SUBJECTED TO HYPOXIA (1% OXYGEN) ON THE INTEGRITY OF THE BLOOD-BRAIN BARRIER, USING BOTH IN VITRO AND ANIMAL MODELS. EXPOSURE OF HUMAN-DERIVED BRAIN ENDOTHELIAL CELL MONOLAYERS TO PLASMA AND PLASMA-DERIVED SMALL EXTRACELLULAR VESICLES FROM PREECLAMPTIC PREGNANCIES INCREASED THE PERMEABILITY AND REDUCED THE TRANSENDOTHELIAL ELECTRICAL RESISTANCE. A SIMILAR OUTCOME WAS OBSERVED WITH HYPOXIC PLACENTAL-DERIVED SMALL EXTRACELLULAR VESICLES, WHICH ALSO INCREASED THE PERMEABILITY TO EVAN?S BLUE IN THE BRAIN OF C57BL6 NONPREGNANT MICE. COTREATMENT WITH MAGNESIUM SULFATE REVERSED THE EFFECTS ELICITED BY PLASMA, PLASMA-DERIVED, AND HYPOXIC PLACENTAL-DERIVED SMALL EXTRACELLULAR VESICLES IN THE EMPLOYED MODELS. THUS, CIRCULATING SMALL EXTRACELLULAR VESICLES IN PLASMA FROM WOMEN WITH PREECLAMPSIA OR FROM HYPOXIC PLACENTAE DISRUPT THE BLOOD-BRAIN BARRIER, WHICH CAN BE PREVENTED USING MAGNESIUM SULFATE. THESE FINDINGS PROVIDE NEW INSIGHTS INTO THE PATHOPHYSIOLOGY OF CEREBRAL COMPLICATIONS ASSOCIATED WITH PREECLAMPSIA.
dc.formatapplication/pdf
dc.identifier.doi10.1161/HYPERTENSIONAHA.121.17744
dc.identifier.issn1524-4563
dc.identifier.issn0194-911X
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/11796
dc.languagespa
dc.publisherHYPERTENSION
dc.relation.uri10.1161/HYPERTENSIONAHA.121.17744
dc.rightsPUBLICADA
dc.titleDISRUPTION OF THE BLOOD-BRAIN BARRIER BY EXTRACELLULAR VESICLES FROM PREECLAMPSIA PLASMA AND HYPOXIC PLACENTAE: ATTENUATION BY MAGNESIUM SULFATE
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS DE REHABILITACION EN SALUD
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
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