Publicación:
SCREENING AND IDENTIFICATION OF COASTAL CHILEAN THRAUSTOCHYTRIDS FOR ARACHIDONIC ACID PRODUCTION: BIOTECHNOLOGICAL POTENTIAL OF ULKENIA VISURGENSIS LNG2-STRAIN

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dc.creatorCLAUDIA ANDREA OVIEDO SILVA
dc.creatorPATRICIA EUGENIA PAULINA ARANCIBIA ÁVILA
dc.creatorJOSÉ ARTURO NAVARRETE ARAYA
dc.date2023
dc.date.accessioned2025-01-10T15:35:15Z
dc.date.available2025-01-10T15:35:15Z
dc.date.issued2023
dc.description.abstractTHRAUSTOCHYTRIDS ARE UNICELLULAR HETEROTROPHIC MARINE PROTISTS THAT HAVE BEEN DESCRIBED AS PRODUCING A HIGH CONTENT OF POLYUNSATURATED FATTY ACIDS (PUFAS). AMONG THEM, ARACHIDONIC ACID (ARA) STANDS OUT AS A PRECURSOR OF SEVERAL MEDIATORS OF PIVOTAL IMPORTANCE FOR THE IMMUNE SYSTEM. HOWEVER, THE BIOTECHNOLOGICAL POTENTIAL OF THRAUSTOCHYTRIDS FOR ARA PRODUCTION HAS NOT BEEN DEVELOPED. THE OBJECTIVE OF THIS STUDY IS TO ISOLATE AND IDENTIFY NATIVE STRAINS FROM DIFFERENT CHILEAN COASTAL ENVIRONMENTS AND EVALUATE IN VITRO THE EFFECT OF CULTURE PARAMETERS SUCH AS C/N RATIO (19 AND 33) AND TEMPERATURE (15 °C AND 23 °C) ON BIOMASS PRODUCTION AND ARACHIDONIC ACID CONTENT. A TOTAL OF NINE STRAINS WERE IDENTIFIED AND CLASSIFIED INTO FOUR GENERA OF THE THRAUSTOCHITRIDAE FAMILY. THE LNG2 STRAIN WITH 99% IDENTITY BELONGS TO THE SPECIES ULKENIA VISURGENIS AND WAS THE MOST PROMINENT ONE FOR ARA PRODUCTION. TEMPERATURE HAD AN EFFECT ON THE PUFA PROFILE BUT NOT ON THE ARA CONTENT NOR ON THE BIOMASS YIELD. ADDITIONALLY, THE C/N RATIO HAS BEEN IDENTIFIED AS A KEY PARAMETER. THE ARA PRODUCTIVITY INCREASED BY 92% (FROM 0.6 TO 8.3 ARA MG/G-DW) AND ITS TOTAL BIOMASS BY 62.7% (FROM 1.9 TO 5.1 G/L) AT A HIGH C/N RATIO (33) AS COMPARED TO THE CONTROL.
dc.formatapplication/pdf
dc.identifier.doi10.3390/microorganisms11030559
dc.identifier.issn2076-2607
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/12717
dc.languagespa
dc.publisherMICROORGANISMS
dc.relation.uri10.3390/microorganisms11030559
dc.rightsPUBLICADA
dc.subjectvery long chain unsaturated fatty acids
dc.subjectUlkenia visurgesis
dc.subjectthraustochytrids
dc.subjectculture parameters
dc.subjectarachidonic acid
dc.titleSCREENING AND IDENTIFICATION OF COASTAL CHILEAN THRAUSTOCHYTRIDS FOR ARACHIDONIC ACID PRODUCTION: BIOTECHNOLOGICAL POTENTIAL OF ULKENIA VISURGENSIS LNG2-STRAIN
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE QUIMICA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.Otra ReparticionDEPARTAMENTO DE INGENIERIA EN MADERAS
ubb.SedeCONCEPCIÓN
ubb.SedeCHILLÁN
ubb.SedeCONCEPCIÓN
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