Publicación: EXPOSURE TO UV-B RADIATION LEADS TO INCREASED DEPOSITION OF CELL WALL-ASSOCIATED XEROCOMIC ACID IN CULTURES OF SERPULA HIMANTIOIDES
Fecha
2019
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Resumen
MANY FUNGI ARE THOUGHT TO HAVE DEVELOPED MORPHOLOGICAL AND PHYSIOLOGICAL ADAPTATIONS TO COPE WITH EXPOSURE TO UV-B RADIATION, BUT IN MOST SPECIES, SUCH RESPONSES AND THEIR PROTECTIVE EFFECTS HAVE NOT BEEN EXPLORED. HERE, WE STUDY THE ADAPTIVE RESPONSE TO UV-B RADIATION IN THE WIDESPREAD, SAPROTROPHIC FUNGUS SERPULA HIMANTIOIDES, FREQUENTLY FOUND COLONIZING CONIFEROUS WOOD IN NATURE. WE REPORT THE MORPHOLOGICAL AND CHEMICAL RESPONSES OF S. HIMANTIOIDES TO CONTROLLED INTENSITIES OF UV-B RADIATION, UNDER IN VITRO CULTURE CONDITIONS. ULTRAVIOLET RADIATION INDUCED A DECREASE IN THE GROWTH RATE OF S. HIMANTIOIDES BUT DID NOT CAUSE GROSS MORPHOLOGICAL CHANGES. INSTEAD, WE OBSERVED ACCUMULATION OF PIGMENTS NEAR THE CELL WALL WITH INCREASING INTENSITIES OF UV-B RADIATION. NUCLEAR MAGNETIC RESONANCE (NMR) AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (HPLC-MS) ANALYSES REVEALED THAT XEROCOMIC ACID WAS THE MAIN PIGMENT PRESENT, BOTH BEFORE AND AFTER UV-B EXPOSURE, INCREASING FROM 7 MG/LITER TO 15 MG/LITER AFTER EXPOSURE. WE SHOW THAT XEROCOMIC ACID IS A PHOTOPROTECTIVE METABOLITE WITH STRONG ANTIOXIDANT ABILITIES, AS EVIDENCED BY DPPH (2,2-DIPHENYL-1-PICRYLHYDRAZYL), ABTS [2,2-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID) DIAMMONIUM SALT], AND OXYGEN RADICAL ABSORBANCE CAPACITY (ORAC) ASSAYS. FINALLY, WE ASSESSED THE CAPACITY OF XEROCOMIC ACID AS A PHOTOPROTECTIVE AGENT ON HEK293 CELLS AND OBSERVED BETTER PHOTOPROTECTIVE PROPERTIES THAN THOSE OF ?-CAROTENE. XEROCOMIC ACID IS THEREFORE A PROMISING NATURAL PRODUCT FOR DEVELOPMENT AS A UV-PROTECTIVE INGREDIENT IN COSMETIC AND PHARMACEUTICAL PRODUCTS.
