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IN VITRO ANTIOXIDANT AND ANTIPROLIFERATIVE EFFECT OF THE EXTRACTS OF EPHEDRA CHILENSIS K PRESL AERIAL PARTS

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dc.creatorENRIQUE ADALBERTO WERNER NAVARRETE
dc.date2019
dc.date.accessioned2025-01-10T15:11:52Z
dc.date.available2025-01-10T15:11:52Z
dc.date.issued2019
dc.description.abstractBACKGROUND: EPHEDRA CHILENSIS K PRESL, KNOWN LOCALLY AS PINGO-PINGO, IS A CHILEAN ENDEMIC PLANT USED IN TRADITIONAL MEDICINE AS AN ANTI-INFLAMMATORY AND USED IN OTHER TREATMENTS. HOWEVER, UNLIKE FOR THE OTHER EPHEDRA SPECIES, THERE HAVE BEEN NO REPORTS ON THE ANTIOXIDANT AND CYTOTOXIC EFFECTS OF THIS PLANT. THE PRESENT STUDY AIMS TO EXPLORE THE POTENTIAL APPLICATIONS OF E. CHILENSIS EXTRACT AS A CYTOTOXIC AGENT AGAINST IN VITRO CANCER CELL LINES AND TO EXPLORE THE RELATIONSHIP BETWEEN THIS EXTRACT AND ANTIOXIDANT ACTIVITY. METHODS: TOTAL ANTHRAQUINONE, FLAVONOID, AND PHENOLIC CONTENTS, AS WELL AS ANTIOXIDANT ACTIVITY (DPPH, FRAP, AND TRAP ASSAYS) AND CYTOTOXIC EFFECT ON SEVERAL CANCER CELL LINES (MCF-7, PC-3, DU-145, AND HT-29) WERE MEASURED FOR THE HEXANE, DICHLOROMETHANE AND ETHANOL EXTRACTS OF E. CHILENSIS. IN ADDITION, SEVERAL CORRELATIONS AMONG THE PHYTOCHEMICAL CONTENT, ANTIOXIDANT ACTIVITY, AND CYTOTOXIC EFFECT WERE EVALUATED. FINALLY, GC-MS ANALYSES OF THE MOST ACTIVE EXTRACTS WERE CARRIED OUT TO IDENTIFY THEIR MAJOR COMPONENTS AND TO RELATE THESE COMPONENTS TO THE CYTOTOXIC EFFECT. RESULTS: ANTIOXIDANT ACTIVITY WAS FOUND IN THE ETOH EXTRACTS OF EPHEDRA, AND THE RESULTS WERE CORRELATED WITH THE PHENOLIC CONTENT. FOR THE CYTOTOXIC ACTIVITY, THE NON-POLAR EXTRACTS OF E. CHILENSIS HAD THE HIGHEST ANTIPROLIFERATIVE EFFECT FOR THE MCF-7 AND PC-3 CANCER LINES; THE EXTRACT WAS SHOWN TO BE UP TO THREE TIMES MORE SELECTIVE THAN DOXORUBICIN. HOWEVER, THE CYTOTOXIC EFFECT WAS NOT CORRELATED WITH THE ANTIOXIDANT ACTIVITY. LASTLY, THE GC-MS ANALYSIS SHOWED A HIGH CONCENTRATION OF SATURATED FATTY ACIDS (MAINLY N-HEXADECANOIC ACID) AND TERPENOIDS (MAINLY 4-(HYDROXY-ETHYL)-?-BUTANOLACTONE).
dc.formatapplication/pdf
dc.identifier.doi10.1186/s12906-019-2462-3
dc.identifier.issn1472-6882
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/10876
dc.languagespa
dc.publisherBMC Complementary and Alternative Medicine
dc.relation.uri10.1186/s12906-019-2462-3
dc.rightsPUBLICADA
dc.titleIN VITRO ANTIOXIDANT AND ANTIPROLIFERATIVE EFFECT OF THE EXTRACTS OF EPHEDRA CHILENSIS K PRESL AERIAL PARTS
dc.title.alternativeEFECTO ANTIOXIDANTE Y ANTIPROLIFERATIVO IN VITRO DE LOS EXTRACTOS DE EPHEDRA CHILENSIS K PRESL PARTES AÉREAS
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
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