Publicación:
DIRECT MEASUREMENT OF INTERACTION FORCES BETWEEN BOVINE SERUM ALBUMIN AND POLY (ETHYLENE OXIDE) IN WATER AND ELECTROLYTE SOLUTIONS

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dc.creatorSERGIO MIGUEL ACUÑA NELSON
dc.creatorJOSÉ MIGUEL BASTÍAS MONTES
dc.date2017
dc.date.accessioned2025-01-10T14:55:16Z
dc.date.available2025-01-10T14:55:16Z
dc.date.issued2017
dc.description.abstractTHE NET INTERACTION BETWEEN A PROBE TIP COATED WITH BOVINE SERUM ALBUMIN (BSA) PROTEIN AND A FLAT SUBSTRATE COATED WITH POLY(ETHYLENE OXIDE) (PEO) POLYMER WAS MEASURED DIRECTLY ON APPROACH IN WATER AND ELECTROLYTE SOLUTIONS USING AFM. THE APPROACH FORCE CURVE BETWEEN THE TWO SURFACES WAS MONOTONICALLY REPULSIVE IN WATER AND IN ELECTROLYTE SOLUTIONS. AT PH ~5, SLIGHTLY ABOVE THE ISOELECTRIC POINT (PI) OF BSA, AND AT LARGE DISTANCES, THE FORCE WAS DOMINATED BY ELECTROSTATIC REPULSION BETWEEN THE OXYGEN ATOMS OF THE INCOMING PROTEIN WITH THOSE BELONGING TO THE ETHER GROUPS OF PEO. SUCH REPULSIVE FORCE AND RANGE DECREASED IN NACL. UNDER PHYSIOLOGICAL CONDITIONS, PH 6, BSA IS DEFINITELY CHARGED AND THE ELECTROSTATIC REPULSION WITH ETHER GROUPS IN PEO APPEARS AT LARGER SEPARATION DISTANCES. INTERESTINGLY, AT PH 4, BELOW THE PI OF BSA, THE REPULSION DECREASED BECAUSE OF AN ATTRACTIVE, ALTHOUGH WEAK, ELECTROSTATIC FORCE THAT APPEARED BETWEEN THE ETHER GROUPS IN PEO AND THE POSITIVELY CHARGED AMINO GROUPS OF BSA. HOWEVER, FOR ALL SOLUTION CONDITIONS, ONCE COMPRESSION OF PEO BEGUN, THE NET REPULSION WAS ALWAYS DOMINATED BY SHORT-RANGE POLYMERIC STERIC REPULSION AND REPULSIVE ENTHALPY PENALTIES FOR BREAKING PEO-WATER BONDS. RESULTS SUGGEST THAT PEO IN MUSHROOM CONFORMATION MAY ALSO BE EFFECTIVE IN REDUCING BIOFOULING.
dc.formatapplication/pdf
dc.identifier.doi10.1371/journal.pone.0173910
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/9562
dc.languagespa
dc.publisherPLoS One
dc.relation.uri10.1371/journal.pone.0173910
dc.rightsPUBLICADA
dc.titleDIRECT MEASUREMENT OF INTERACTION FORCES BETWEEN BOVINE SERUM ALBUMIN AND POLY (ETHYLENE OXIDE) IN WATER AND ELECTROLYTE SOLUTIONS
dc.title.alternativeMEDICIÓN DIRECTA DE LAS FUERZAS DE INTERACCIÓN ENTRE LA ALBÚMINA SÉRICA BOVINA Y EL POLI(ÓXIDO DE ETILENO) EN SOLUCIONES DE AGUA Y ELECTROLITOS
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDIRECCION DE INVESTIGACION
ubb.Otra ReparticionDEPARTAMENTO DE INGENIERIA EN ALIMENTOS
ubb.SedeCONCEPCIÓN
ubb.SedeCHILLÁN
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