Publicación:
FROM TRADITIONAL TYPING TO GENOMIC PRECISION: WHOLE-GENOME SEQUENCING OF LISTERIA MONOCYTOGENES ISOLATED FROM REFRIGERATED FOODS IN CHILE

Vista sencilla de ítem
dc.creatorJULIO ENRIQUE PARRA FLORES
dc.creatorPAMELA ANDREA CHAVARRÍA SEPÚLVEDA
dc.date2025
dc.date.accessioned2025-12-15T17:57:08Z
dc.date.available2025-12-15T17:57:08Z
dc.date.issued2025
dc.description.abstractREADY-TO-EAT (RTE) FOODS ARE THE MOST COMMON SOURCES OF LISTERIA MONOCYTOGENES TRANSMISSION. WHOLE-GENOME SEQUENCING (WGS) ENHANCES THE INVESTIGATION OF FOODBORNE OUTBREAKS BY ENABLING THE TRACKING OF PATHOGEN SOURCES AND THE PREDICTION OF GENETIC TRAITS RELATED TO VIRULENCE, STRESS, AND ANTIMICROBIAL RESISTANCE, WHICH BENEFIT FOOD SAFETY MANAGEMENT. THE AIM OF THIS STUDY WAS TO EVALUATE THE EFFICACY OF WGS IN THE TYPING OF 16 L. MONOCYTOGENES STRAINS ISOLATED FROM REFRIGERATED FOODS IN CHILE, HIGHLIGHTING ITS ADVANTAGES IN PATHOGEN IDENTIFICATION AND THE IMPROVEMENT OF EPIDEMIOLOGICAL SURVEILLANCE AND FOOD SAFETY. USING CGMLST, A CLUSTER WAS IDENTIFIED COMPRISING 2 STRAINS WITH ZERO ALLELE DIFFERENCES AMONG THE 16 STRAINS EVALUATED. NINETY-FOUR PERCENT OF THE ISOLATES (15/16) WERE SEROTYPE 1/2B, AND 88% OF THEM (14/16) WERE ST5. ALL STRAINS SHARED IDENTICAL VIRULENCE GENES RELATED TO ADHESION (AMI, IAP, LAPB), STRESS RESISTANCE (CLPCEP), INVASION (AUT, IAPCWHA, INLAB, LPEA), TOXIN PRODUCTION (HLY), AND INTRACELLULAR REGULATION (PRFA), WITH ONLY 13 STRAINS EXHIBITING THE BCRBC AND QACJ GENE, WHICH CONFER RESISTANCE TO QUATERNARY AMMONIUM. THE PCFSAN010068_01 PLASMIDS WERE PREVALENT, AND INSERTION SEQUENCES (ISLS) AND COMPOSITE TRANSPOSONS (CNS) WERE DETECTED IN 87.5% OF THE STRAINS. THE PRESENCE OF VARIOUS ANTIBIOTIC RESISTANCE GENES, ALONG WITH RESISTANCE TO THERMAL SHOCKS AND DISINFECTANTS, MAY PROVIDE L. MONOCYTOGENES ST5 STRAINS WITH ENHANCED ENVIRONMENTAL RESISTANCE TO THE HYGIENE TREATMENTS USED IN THE STUDIED FOOD PRODUCTION PLANT.
dc.formatapplication/pdf
dc.identifier.doi10.3390/foods14020290
dc.identifier.issn2304-8158
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/14305
dc.language
dc.publisherFOODS
dc.relation.uri10.3390/foods14020290
dc.rightsOPEN ACCESS
dc.subjectListeria monocytogenes
dc.subjectWhole-genome sequencing
dc.subjectAntimicrobial resistance genes
dc.subjectVirulence genes
dc.titleFROM TRADITIONAL TYPING TO GENOMIC PRECISION: WHOLE-GENOME SEQUENCING OF LISTERIA MONOCYTOGENES ISOLATED FROM REFRIGERATED FOODS IN CHILE
dc.typeARTÍCULO
dspace.entity.typePublication
oaire.licenseConditionCC BY 4.0
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE NUTRICION Y SALUD PUBLICA
ubb.Otra ReparticionDEPARTAMENTO DE NUTRICION Y SALUD PUBLICA
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
Archivos
Colecciones
Artículos científicos