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HYPOXIC PLACENTAL SMALL EXTRACELLULAR VESICLES DISRUPT THE BLOOD-BRAIN BARRIER IN THE FRONTAL AREA OF THE BRAIN

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dc.creatorJESENIA MARIANELA ACURIO JÁCOME
dc.creatorBELÉN OCTAVIA DE LOS ÁNGELES IBÁÑEZ JARA
dc.creatorHERMES SEBASTIÁN SANDOVAL RIVAS
dc.creatorCARLOS ALONSO ESCUDERO OROZCO
dc.date2022
dc.date.accessioned2025-01-10T15:32:35Z
dc.date.available2025-01-10T15:32:35Z
dc.date.issued2022
dc.description.abstractOBJECTIVES: INVESTIGATE WHETHER SMALL EXTRACELLULAR VESICLES (PDSEVS) EXTRACTED FROM PLACENTAS CULTURED IN HYPOXIA DISRUPT THE BLOOD-BRAIN BARRIER (BBB) IN SELECTED AREAS OF THE BRAIN. METHODS: PDSEVSWERE EXTRACTED FROM PLACENTAL EXPLANTS OF NORMAL PREGNANCY CULTURED IN NORMOXIA (21% O2) OR HYPOXIA (1% O2, 18 H). PDSEVS (100 MG OF PROTEIN) FROM NORMOXIA (N=6) OR HYPOXIA (N=6) WERE INJECTED IN NON-PREGNANT FEMALE C57BL/7 MICE. INJECTED MICE WERE EVALUATED USING THE RAPID MURINE COMA AND BEHAVIOR SCALE (RMSBS, 0-24 H), WHILE SYSTEMIC BLOOD PRESSURE WAS MEASURED BEFORE AND AFTER TREATMENT. MICE WERE INJECTED WITH EVAN S BLUE AND THEN EUTHANIZED TO EXTRACT THE BRAIN. MICE BRAINS WERE WEIGHED AND SECTIONED (100 MM SLICES) TO ANALYZE EVAN S BLUE EXTRAVASATION. ALSO, HUMAN CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS (HCMEC/D3) WERE EXPOSED TO PDSEVS. BBB PARAMETERS INCLUDED ELECTRICAL RESISTANCE (TEER) AND CELL PERMEABILITY TO A HIGH-MOLECULAR-WEIGHT FLUORESCENT DYE. ALSO, THE AMOUNT OF CLAUDIN-5 IN THE CELL MEMBRANE WAS ANALYZED BY WESTERN BLOT. UPTAKE OF PDSEVS WAS ANALYZED USING PKH67. RESULTS: MICE TREATED WITH HYPOXIC PDSEVS EXHIBITED NEUROLOGICAL DEFICITS (FROM 3 TO 24 H), AS INDICATED WITH A SIGNIFICANT REDUCTION IN THE SCORE FOR SPINAL REFLEXES AND MOTOR SKILLS, WHICH WAS ASSOCIATED WITH MORE SIGNIFICANT EXTRAVASATION OF EVAN S BLUE, MAINLY IN THE ANTERIOR PORTION OF THE BRAIN. THERE WERE NO SIGNIFICANT CHANGES IN SYSTOLIC/DIASTOLIC BLOOD PRESSURE IN ANY OF THE TREATED GROUPS. HYPOXIC PDSEVS GENERATED A MORE SIGNIFICANT REDUCTION IN THE TEER VALUES (P
dc.formatapplication/pdf
dc.identifier.doi10.1016/j.placenta.2022.03.054
dc.identifier.issn1532-3102
dc.identifier.issn0143-4004
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/12509
dc.languagespa
dc.publisherPLACENTA
dc.relation.uri10.1016/j.placenta.2022.03.054
dc.rightsPUBLICADA
dc.titleHYPOXIC PLACENTAL SMALL EXTRACELLULAR VESICLES DISRUPT THE BLOOD-BRAIN BARRIER IN THE FRONTAL AREA OF THE BRAIN
dc.typeMEETING ABSTRACT
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS DE REHABILITACION EN SALUD
ubb.Otra ReparticionDIRECCION DE INNOVACION
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
ubb.SedeCONCEPCIÓN
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
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