Publicación:
CALCIUM-SENSING RECEPTOR IN ADIPOSE TISSUE: POSSIBLE ASSOCIATION WITH OBESITY-RELATED ELEVATED AUTOPHAGY

Vista sencilla de ítem
dc.creatorLAUTARO RIGOBERTO NABI BRIONES SUÁREZ
dc.date2020
dc.date.accessioned2025-01-10T15:15:49Z
dc.date.available2025-01-10T15:15:49Z
dc.date.issued2020
dc.description.abstractAUTOPHAGY IS UPREGULATED IN ADIPOSE TISSUE (AT) FROM PEOPLE WITH OBESITY. WE SHOWED THAT ACTIVATION OF THE CALCIUM-SENSING RECEPTOR (CASR) ELEVATES PROINFLAMMATORY CYTOKINES THROUGH AUTOPHAGY IN PREADIPOCYTES. OUR AIM IS TO UNDERSTAND THE ROLE OF CASR ON AUTOPHAGY IN AT FROM HUMANS WITH OBESITY. WE DETERMINED MRNA AND PROTEIN LEVELS OF CASR AND MARKERS OF AUTOPHAGY BY QPCR AND WESTERN BLOT IN HUMAN VISCERAL AT EXPLANTS OR ISOLATED PRIMARY PREADIPOCYTES (60 DONORS: 72% FEMALE, 23-56% BODY FAT). WE ALSO INVESTIGATED THEIR ASSOCIATION WITH DONORS ANTHROPOMETRIC VARIABLES. DONORS % BODY FAT AND CASR MRNA EXPRESSION IN AT WERE CORRELATED (R = 0.44, P < 0.01). CASR EXPRESSION WAS ASSOCIATED WITH MRNA LEVELS OF THE AUTOPHAGY MARKERS ATG5 (R = 0.37, P < 0.01), ATG7 (R = 0.29, P < 0.05) AND LC3B (R = 0.40, P < 0.01). CASR ACTIVATION INCREASED BECN AND ATG7 MRNA EXPRESSION IN AT. CASR ACTIVATION ALSO UPREGULATED LC3II BY ~50%, AN EFFECT ABOLISHED BY THE CASR INHIBITOR. SPERMINE (CASR AGONIST) REGULATES LC3II THROUGH THE ERK1/2 PATHWAY. STRUCTURAL EQUATION MODEL ANALYSIS SUGGESTS A LINK BETWEEN DONORS AT CASR EXPRESSION, AT AUTOPHAGY AND EXPRESSION OF TUMOR NECROSIS FACTOR ALPHA TNF-?. CASR EXPRESSION IN VISCERAL AT IS DIRECTLY ASSOCIATED WITH % BODY FAT, AND CASR ACTIVATION MAY CONTRIBUTE TO OBESITY-RELATED DISRUPTION IN AT AUTOPHAGY.
dc.formatapplication/pdf
dc.identifier.doi10.3390/ijms21207617
dc.identifier.issn1422-0067
dc.identifier.issn1422-0067
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/11185
dc.languagespa
dc.publisherINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
dc.relation.uri10.3390/ijms21207617
dc.rightsPUBLICADA
dc.titleCALCIUM-SENSING RECEPTOR IN ADIPOSE TISSUE: POSSIBLE ASSOCIATION WITH OBESITY-RELATED ELEVATED AUTOPHAGY
dc.title.alternativeRECEPTOR SENSIBLE AL CALCIO EN EL TEJIDO ADIPOSO: POSIBLE ASOCIACIÓN CON LA AUTOFAGIA ELEVADA RELACIONADA CON LA OBESIDAD
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE NUTRICION Y SALUD PUBLICA
ubb.SedeCHILLÁN
Archivos
Colecciones
Artículos científicos