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BIOACTIVE EXTRACTS FROM GRAPE (VITIS VINIFERA L. CV. PAIS) LEAVES: CHARACTERIZATION AND ENCAPSULATION IN POLY-3-HYDROXYBUTYRATE-CO-3-VALERATE MICROPARTICLES

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dc.creatorEDGAR RAFAEL PASTENE NAVARRETE
dc.date2024
dc.date.accessioned2025-01-10T15:48:04Z
dc.date.available2025-01-10T15:48:04Z
dc.date.issued2024
dc.description.abstractVITIS VINIFERA VAR. L. PAÍS LEAF EXTRACT, PREVIOUSLY CHARACTERIZED, WAS MICROENCAPSULATED BY EMULSION-SOLVENT EVAPORATION METHOD USING TWO DIFFERENT PROCEDURES: THE CONVENTIONAL DROPWISE AND MICROMIXER DEVICE. THE POLY-3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE (PHBV) WAS USED AS ENCAPSULATING POLYMER. IN ADDITION, THE EFFECT OF EXTRACTION TIME AND ETHANOL:WATER RATIO ON THE YIELD AND THE CONTENT OF BIOACTIVE COMPOUNDS OF GRAPE LEAF EXTRACT OBTAINED BY SOXHLET METHOD WAS DETERMINED USING THE FACE-CENTERED CENTRAL COMPOSITE DESIGN. THE HIGHEST BIOACTIVE COMPOUND WERE OBTAINED USING A 70:30 ETHANOL:WATER SOLVENT RATIO DURING 4 CYCLES. THIS EXTRACT IS MAINLY COMPOSED OF CINNAMIC ACID, QUERCETIN-3-GLUCOSIDE AND QUERCETIN-3-GLUCORINIDE, CONTAINING 3.56, 2.08 AND 1.19 MG G?1 EXTRACT, RESPECTIVELY. THE TOTAL POLYPHENOL CONTENT DETERMINED BY FOLIN CIOCALTEU METHOD WAS 164 ± 14 MG GAE G?1 EXTRACT. THE MICROPARTICLES (2?3 ?M) HAD SPHERICAL SHAPE WITH ROUGH AND POROUS SURFACE. THE ENCAPSULATION EFFICIENCY WAS 15.1% AND 60.6% FOR THE PHBV MICROPARTICLES PREPARED USING CONVENTIONAL DROPWISE AND MICROMIXER DEVICE, RESPECTIVELY. THE ANTIOXIDANT ACTIVITY PRESENT IN THE ORIGINAL EXTRACT WAS PRESERVED AROUND 50%?80% AND 20%?70% IN THE LOADED MICROPARTICLES. THE BEST RESULTS WERE ACHIEVED WHEN GRAPE LEAF EXTRACT WAS ENCAPSULATED USING MICROMIXER DEVICE. THE CELL VIABILITY ASSAY IN 3T3-L1 FIBROBLASTS, SHOWED THAT LOADED MICROPARTICLES ARE NOT CYTOTOXIC. IN CONCLUSION MICROMIXER DEVICE SHOWED TO BE EFFECTIVE FOR THE MICROENCAPSULATION OF GRAPE LEAF EXTRACT USING PHBV AS ENCAPSULING POLYMER.
dc.formatapplication/pdf
dc.identifier.doi10.1016/j.fbio.2024.103968
dc.identifier.issn2212-4306
dc.identifier.issn2212-4292
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/13712
dc.languagespa
dc.publisherFOOD BIOSCIENCE
dc.relation.uri10.1016/j.fbio.2024.103968
dc.rightsPUBLICADA
dc.subjectQUERCETIN
dc.subjectMICROSPHERES
dc.subjectANTIOXIDANT ACTIVITY
dc.titleBIOACTIVE EXTRACTS FROM GRAPE (VITIS VINIFERA L. CV. PAIS) LEAVES: CHARACTERIZATION AND ENCAPSULATION IN POLY-3-HYDROXYBUTYRATE-CO-3-VALERATE MICROPARTICLES
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
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