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POTENTIAL ROLE OF A2B ADENISINE RECEPTORS ON PROLIFERATION/MEGRATION OF FETAL ENDOTHELIUM DERIVED FROM PRE-ECLAMPTIC PREGNANCIES OF FETAL ENDOTHELIUM DERIVED FROM PRE-ECLAMTIC PREGNANCIES.

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dc.creatorJESENIA MARIANELA ACURIO JÁCOME
dc.creatorFELIPE ANDRÉS TRONCOSO BASSO
dc.creatorCARLOS ALONSO ESCUDERO OROZCO
dc.date2014
dc.date.accessioned2025-01-10T14:47:58Z
dc.date.available2025-01-10T14:47:58Z
dc.date.issued2014
dc.description.abstractTO INVESTIGATE THE FUNCTIONALITY OF A2B ADENOSINE RECEPTOR (A2BAR) AND THE NITRIC OXIDE (NO) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) SIGNALING PATHWAY IN THE ENDOTHELIAL CELL PROLIFERATION/MIGRATION DURING PREECLAMPSIA, WE USED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECS) ISOLATED FROM NORMAL PREGNANCIES (N = 15) OR PREGNANCIES WITH PREECLAMPSIA (N = 15). EXPERIMENTS WERE PERFORMED IN PRESENCE OR ABSENCE OF THE NONSELECTIVE ADENOSINE RECEPTOR AGONIST NECA, THE A2BAR SELECTIVE ANTAGONIST MRS-1754, AND THE NITRIC OXIDE SYNTHASE (NOS) INHIBITOR L-NAME. RESULTS INDICATED THAT CELLS FROM PREECLAMPSIA EXHIBITED A SIGNIFICANT HIGHER PROTEIN LEVEL OF A2BAR AND LOGEC50 FOR NECA-MEDIATED PROLIFERATION THAN NORMOTENSIVE PREGNANCIES. THE STIMULATORY EFFECT OF NECA (10??M, 24?H) ON CELL PROLIFERATION WAS PREVENTED BY MRS-1754 (5?NM) COINCUBATION ONLY IN CELLS FROM NORMOTENSIVE PREGNANCIES. NEVERTHELESS, L-NAME (100??M, 24?H) REDUCED THE NECA-INDUCED CELL PROLIFERATION/MIGRATION IN HUVEC FROM NORMAL PREGNANCY; HOWEVER IN PREECLAMPSIA ONLY NECA-INDUCED CELL PROLIFERATION WAS REDUCED BY L-NAME. MOREOVER, NECA INCREASED PROTEIN NITRATION AND ABUNDANCE OF VEGF IN CELLS FROM NORMAL PREGNANCY AND EFFECT PREVENTED BY MRS-1754 COINCUBATION. NEVERTHELESS, IN PREECLAMPSIA NECA DID NOT AFFECT THE PROTEIN LEVEL OF VEGF. IN CONCLUSION HUVECS FROM PREECLAMPSIA EXHIBIT ELEVATED PROTEIN LEVEL OF A2BAR AND IMPAIRMENT OF A2BAR-MEDIATED NO/VEGF SIGNALING PATHWAY.
dc.formatapplication/pdf
dc.identifier.doi10.1155/2014/274507
dc.identifier.issn2314-6141
dc.identifier.issn2314-6133
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/9020
dc.languagespa
dc.publisherBiomed Research International
dc.relation.uri10.1155/2014/274507
dc.rightsPUBLICADA
dc.titlePOTENTIAL ROLE OF A2B ADENISINE RECEPTORS ON PROLIFERATION/MEGRATION OF FETAL ENDOTHELIUM DERIVED FROM PRE-ECLAMPTIC PREGNANCIES OF FETAL ENDOTHELIUM DERIVED FROM PRE-ECLAMTIC PREGNANCIES.
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
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