Publicación:
THE GPI-ANCHORED PROTEIN THY-1/CD90 PROMOTES WOUND HEALING UPON INJURY TO THE SKIN BY ENHANCING SKIN PERFUSION

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dc.creatorJOSÉ MANUEL LEÓN PINO
dc.creatorCARLOS ALONSO ESCUDERO OROZCO
dc.date2022
dc.date.accessioned2025-01-10T15:35:10Z
dc.date.available2025-01-10T15:35:10Z
dc.date.issued2022
dc.description.abstractWOUND HEALING IS A HIGHLY REGULATED MULTI-STEP PROCESS THAT INVOLVES A PLETHORA OF SIGNALS. BLOOD PERFUSION IS CRUCIAL IN WOUND HEALING AND ABNORMALITIES IN THE FORMATION OF NEW BLOOD VESSELS DEFINE THE OUTCOME OF THE WOUND HEALING PROCESS. THY-1 HAS BEEN IMPLICATED IN ANGIOGENESIS AND SILENCING OF THE THY-1 GENE RETARDS THE WOUND HEALING PROCESS. HOWEVER, THE ROLE OF THY-1 IN BLOOD PERFUSION DURING WOUND CLOSURE REMAINS UNCLEAR. WE PROPOSED THAT THY-1 REGULATES VASCULAR PERFUSION, AFFECTING THE HEALING RATE IN MOUSE SKIN. WE ANALYZED THE TIME OF RECOVERY, BLOOD PERFUSION USING LASER SPECKLE CONTRAST IMAGING, AND TISSUE MORPHOLOGY FROM IMAGES ACQUIRED WITH A NANOZOOMER TISSUE SCANNER. THE LATTER WAS ASSESSED IN A TISSUE SAMPLE TAKEN WITH A BIOPSY PUNCH ON SEVERAL DAYS DURING THE WOUND HEALING PROCESS. RESULTS OBTAINED WITH THE THY-1 KNOCKOUT (THY-1-/-) MICE WERE COMPARED WITH CONTROL MICE. THY-1-/- MICE SHOWED AT DAY SEVEN, A DELAYED RE-EPITHELIALIZATION, INCREASED MICRO- TO MACRO-CIRCULATION RATIO, AND LOWER BLOOD PERFUSION IN THE WOUND AREA. IN ADDITION, SKIN MORPHOLOGY DISPLAYED A FLATTER EPIDERMIS, FEWER RIDGES, AND ALMOST NO STRATUM GRANULOSUM OR CORNEUM, WHILE THE DERMIS WAS THICKER, SHOWING MORE FIBROBLASTS AND FEWER LYMPHOCYTES. OUR RESULTS SUGGEST A CRITICAL ROLE FOR THY-1 IN WOUND HEALING, PARTICULARLY IN VASCULAR DYNAMICS.
dc.formatapplication/pdf
dc.identifier.doi10.3390/ijms232012539
dc.identifier.issn1422-0067
dc.identifier.issn1422-0067
dc.identifier.urihttps://repositorio.ubiobio.cl/handle/123456789/12710
dc.languagespa
dc.publisherINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
dc.relation.uri10.3390/ijms232012539
dc.rightsPUBLICADA
dc.subjectWOUND HEALING
dc.subjectTHY-1(CD90)
dc.subjectSKIN INJURIES
dc.subjectFOURIER ANALISYS
dc.subjectBLOOD PERFUSION
dc.subjectANGIOGENESIS
dc.titleTHE GPI-ANCHORED PROTEIN THY-1/CD90 PROMOTES WOUND HEALING UPON INJURY TO THE SKIN BY ENHANCING SKIN PERFUSION
dc.typeARTÍCULO
dspace.entity.typePublication
ubb.EstadoPUBLICADA
ubb.Otra ReparticionFACULTAD DE CIENCIAS DE LA SALUD Y DE LOS ALIMENTOS
ubb.Otra ReparticionDEPARTAMENTO DE CIENCIAS BASICAS
ubb.SedeCHILLÁN
ubb.SedeCHILLÁN
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