Publicación: LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
Fecha
2019
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JOURNAL OF THE CHILEAN CHEMICAL SOCIETY
Resumen
A RAPID, SENSITIVE, PRECISE AND ACCURATE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS) METHOD WAS DEVELOPED FOR LEVO 3,4-DIHYDROXYPHENYLALANINE (L-DOPA) DETERMINATION IN VICIA FABA DURING DIFFERENT GROWTH STAGES. THE METHOD APPLIED A SIMPLE SAMPLE PREPARATION STEP FOLLOWED BY A CHROMATOGRAPHIC SEPARATION ON A KINETEX XB CORE-SHELL C18 (100 MM X 4.6 MM, 2.6 ?M) COLUMN, USING A MIXTURE OF ULTRAPURE PURE WATER (A) WITH 0.5 % (V/V) FORMIC ACID AND METHANOL (B) AS MOBILE PHASE. ANALYSIS OF L-DOPA WAS CARRIED OUT BY MS/MS APPLYING A MULTIPLE REACTION MONITORING (MRM) METHOD USING THE TRANSITION M/Z 198 ? M/Z 152. THIS LC-MS/MS METHOD ALLOWED A WELL-RESOLVED DETECTION OF L-DOPA IN CA. 2 MIN WITHIN 6 MIN RUN. METHOD WAS VALIDATED SHOWING A LINEAR RANGE FROM 0.05 TO 10 MG L-1 (R2 = 0.99); REPEATABILITY SHOWED RSD VALUE OF 1.40%. RECOVERIES RANGED FROM 94.14 TO 116.62% WITH RSD VALUES ? 5.66% AND DETECTION AND QUANTIFICATION LIMITS WERE 0.01 AND 0.05 MG L-1, RESPECTIVELY. APPLYING THIS VALIDATED METHOD, L-DOPA WAS DETERMINED IN VICIA FABA SAMPLES TO DETERMINED ITS TISSUE DISTRIBUTION. AS EXPECTED, A BROAD RANGE OF L-DOPA CONTENT FINDING THE LOWEST CONTENT IN SEEDS (4.72±2.12 MG G-1) AND THE HIGHEST IN SPROUTS (133.60±5.65 MG G-1).
