Publicación: ANTIFUNGAL ACTIVITIES OF SECONDARY METABOLITES ISOLATED FROM LIQUID FERMENTATIONS OF STEREUM HIRSUTUM (SH134-11)AGAINST BOTRYTIS CINEREA (GREY MOULD AGENT)
Fecha
2017
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FOOD AND CHEMICAL TOXICOLOGY
Resumen
EXTRACTS OBTAINED FROM LIQUID MYCELIAL FERMENTATIONS OF THE CHILEAN FUNGUS STEREUM HIRSUTUM (SH134-11) SHOWED ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA. TWO TYPES OF EXTRACTS WERE OBTAINED: ETOAC-EXTRACT (LIQUID PHASE) AND MEOH-EXTRACT (MYCELIAL PHASE). PLATE DIFFUSION ASSAY SHOWED THAT ETOAC-EXTRACTS WERE MORE ACTIVE THAN MEOH-EXTRACTS. A LARGE-SCALE FERMENTATION OF SH134-11 AND CHROMATOGRAPHIC METHODS ALLOWED TO ISOLATED FOUR COMPOUNDS: MS-3, VIBRALACTONE, VIBRALACTONE B AND STERENIN D. ONLY STERENIN D SHOWED ANTIFUNGAL ACTIVITY AGAINST B. CINEREA IN THE TESTS PERFORMED. EFFECTS ON THE MYCELIAL GROWTH OF B. CINEREA SHOWED THAT STERENIN D SHOWED INHIBITION AT 1000-2000 ?G/ML REACHING 67% AND 76% RESPECTIVELY. STERENIN D WAS MORE EFFECTIVE TO CONTROL THE SPOROGENESIS, INHIBITING IN 96% THE SPORULATION AT 500 ?G/ML. ASSAYS SHOWED THAT STERENIN D EXHIBITED A MINIMAL FUNGICIDAL CONCENTRATION (MFC) OF 50 ?G/ML AND MINIMAL INHIBITORY CONCENTRATION (MIC) AT 20 ?G/ML. OUR STUDY INDICATED THAT SUBMERGED FERMENTATIONS OF CHILEAN S. HIRSUTUM (SH134-11) PRODUCED EXTRACTS WITH ANTIFUNGAL ACTIVITY AND STERENIN D IS RESPONSIBLE FOR THIS ACTIVITY, WHICH COULD BE USED AS POSSIBLE BIOFUNGICIDES ALTERNATIVE TO SYNTHETIC FUNGICIDES.
