Publicación: ANTIHYPERGLYCEMIC AFFE AND GENOTOXICITY OF PSITTACANTHUS CALYCULATUS EXTRACT IN STRETOZOTOCIN-INDUCED DIABETIC RATS.
Fecha
2012
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Editor
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
Resumen
PSITTACANTHUS CALYCULATUS (DC.) G. DON (LORANTACEAE) IS KNOWN AS INGERTO. THE AERIAL PARTS ARE USED IN THE TREATMENT OF DIABETES AND HYPERTENSION. METHANOLIC EXTRACT WAS TESTED WITH STREPTOZOTOCIN-INDUCED DIABETIC RATS. DOSE OF 200 MG/KG BODY WEIGHT FOR ACUTE EXPERIMENTS, AS WELL AS 200 AND 400 MG/KG FOR SEMI-CHRONIC BIOASSAY WERE USED. IN BOTH EXPERIMENTS EXTRACT PRODUCED SIGNIFICANT HYPOGLYCEMIC ACTIVITY IN STREPTOZOTOCIN-INDUCED RATS WHEN COMPARED WITH DIABETIC CONTROL (P 0.05). TO STUDY POSSIBLE CLASTOGENIC EFFECTS OF METHANOLIC EXTRACT A MOUSE MICRONUCLEUS TEST WAS PERFORMED (AS PART OF THE GENETIC TOXICOLOGY TRIAL). CD-1 WHITE MICE WERE ADMINISTERED WITH 200 AND 400 MG /KG OF METHANOLIC EXTRACT OF P. CALYCULATUS DISSOLVED IN WATER BY INTRAPERITONEAL INJECTION. THE CYTOTOXIC ACTIVITY POLYCHROMATIC ERYTHROCYTES/NORMOCHROMATIC ERYTHROCYTES (PCE/NCE) AND THE INDUCTION OF MICRONUCLEI IN PERIPHERAL BLOOD ERYTHROCYTES (MNPCE) WAS RECORDED WITH SAMPLING TIMES OF 24, 48 AND 72, H AFTER AN EXPOSURE WITHOUT KILLING OF MICE. THE FREQUENCY OF MNPCE IN THE CIRCULATING BLOOD OBTAINED FROM THE TAIL OF THE MOUSE WAS STATISTICALLY NOT SIGNIFICANT COMPARED WITH ITS NEGATIVE CONTROL ANIMALS (TIME ZERO) AND THE PCE/NCE RATIO SHOWED EVIDENCES OF LIGHT CYTOTOXIC ACTIVITY COMPARED WITH ITS NEGATIVE CONTROL ANIMALS (TIME ZERO). THUS, IN THIS TEST, THE METHANOLIC EXTRACT OF PSITTACANTHUS CALYCULATUS DISSOLVED IN WATER DID NOT INDUCE CHROMOSOMAL DAMAGE RESULTING IN MICRONUCLEUS FORMATION IN PERIPHERAL BLOOD ERYTHROCYTES AND SHOWED LIGHT CYTOTOXIC ACTIVITY.
