Publicación: THE GPI-ANCHORED PROTEIN THY-1/CD90 PROMOTES WOUND HEALING UPON INJURY TO THE SKIN BY ENHANCING SKIN PERFUSION
Fecha
2022
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Resumen
WOUND HEALING IS A HIGHLY REGULATED MULTI-STEP PROCESS THAT INVOLVES A PLETHORA OF SIGNALS. BLOOD PERFUSION IS CRUCIAL IN WOUND HEALING AND ABNORMALITIES IN THE FORMATION OF NEW BLOOD VESSELS DEFINE THE OUTCOME OF THE WOUND HEALING PROCESS. THY-1 HAS BEEN IMPLICATED IN ANGIOGENESIS AND SILENCING OF THE THY-1 GENE RETARDS THE WOUND HEALING PROCESS. HOWEVER, THE ROLE OF THY-1 IN BLOOD PERFUSION DURING WOUND CLOSURE REMAINS UNCLEAR. WE PROPOSED THAT THY-1 REGULATES VASCULAR PERFUSION, AFFECTING THE HEALING RATE IN MOUSE SKIN. WE ANALYZED THE TIME OF RECOVERY, BLOOD PERFUSION USING LASER SPECKLE CONTRAST IMAGING, AND TISSUE MORPHOLOGY FROM IMAGES ACQUIRED WITH A NANOZOOMER TISSUE SCANNER. THE LATTER WAS ASSESSED IN A TISSUE SAMPLE TAKEN WITH A BIOPSY PUNCH ON SEVERAL DAYS DURING THE WOUND HEALING PROCESS. RESULTS OBTAINED WITH THE THY-1 KNOCKOUT (THY-1-/-) MICE WERE COMPARED WITH CONTROL MICE. THY-1-/- MICE SHOWED AT DAY SEVEN, A DELAYED RE-EPITHELIALIZATION, INCREASED MICRO- TO MACRO-CIRCULATION RATIO, AND LOWER BLOOD PERFUSION IN THE WOUND AREA. IN ADDITION, SKIN MORPHOLOGY DISPLAYED A FLATTER EPIDERMIS, FEWER RIDGES, AND ALMOST NO STRATUM GRANULOSUM OR CORNEUM, WHILE THE DERMIS WAS THICKER, SHOWING MORE FIBROBLASTS AND FEWER LYMPHOCYTES. OUR RESULTS SUGGEST A CRITICAL ROLE FOR THY-1 IN WOUND HEALING, PARTICULARLY IN VASCULAR DYNAMICS.
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Palabras clave
WOUND HEALING, THY-1(CD90), SKIN INJURIES, FOURIER ANALISYS, BLOOD PERFUSION, ANGIOGENESIS
