Publicación: ELEVATED PRO-ANGIOGENIC PHENOTYPE IN FETO-PLACENTAL TISSUE FROM GESTATIONAL DIABETES MELLITUS
Fecha
2015
Título de la revista
ISSN de la revista
Título del volumen
Editor
PLACENTA
Resumen
OBJECTIVE: WE AIMED TO INVESTIGATE WHETHER FETO-PLACENTAL TISSUE FROM GESTATIONAL DIABETES MELLITUS (GDM) EXHIBITS AN ELEVATED PRO-ANGIOGENIC PHENOTYPE COMPARED TO NORMAL PREGNANCY.
METHODS: WE OBTAINED PLACENTAL SAMPLES FROM NORMAL PREGNANT WOMEN (N=12) AND GDM PATIENTS (N=14), WHICH WERE HOMOGENIZED TO OBTAIN MRNA AND PROTEIN EXTRACTIONS. SEMI-QUANTITATIVE PCR WAS PERFORMED TO ESTIMATE MRNA LEVELS OF VEGF, VEGFR TYPE 1 (VEGFR1) AND VEGFR2. ALSO, PROTEIN CONCENTRATIONS OF CD31, CD34, KI67, VEGF, VEGFR1, VEGFR2 AND Y951-PHOSPHORYLATED VEGFR2 WERE ESTIMATED BY WESTERN BLOT. Y1175 PHOSPHORYLATED VEGFR2 WAS MEASURED BY ELISA. ALSO HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVEC) WERE ISOLATED FROM NORMAL (N=10) AND GDM (N=10) WOMAN. CELL PROLIFERATION WAS ANALYZED BY COLORIMETRIC MTS-ASSAY (PROMEGA, USA), WHEREAS MIGRATION WAS ANALYZED BY CELL-SCRATCH ASSAY AFTER 24 HOURS OF CELL INCUBATION WITHOUT ANY TREATMENT.
