Publicación: TYROSINASE INHIBITORS FROM CALCEOLARIA INTEGRIFOLIA S.L.: CALCEOLARIA TALCANA AERIAL PARTS
Fecha
2013
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Editor
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Resumen
AS A DEFENSE MECHANISM OF THE AERIAL PARTS OF CALCEOLARIA TALCANA (CALCEOLARIACEAE; FORMERLY SCROPHULARIACEAE) AGAINST HERBIVORE OFFENSES AND INSECT PEST ATTACK, DITERPENOIDS, TRITERPENOIDS, PHENYLETHANOIDS, FLAVONOIDS, AND IRIDOIDS ARE RAPIDLY ACCUMULATED ALONG THE AERIAL PARTS, RESULTING IN A UNIQUE NATURAL BIOPESTICIDE COMPLEX FROM THIS PLANT. IN ADDITION TO VERBASCOSIDE A SERIES OF KNOWN COMPOUNDS WERE SCREENED FOR THEIR INHIBITORY ACTIVITY AGAINST MUSHROOM TYROSINASE AND PROTEASE ENZYMES. ETHYL ACETATE AND N-HEXANE EXTRACTS, TOGETHER WITH CYCLOPROPYL-7,15-ENT-PIMARADIENE (1), ABIETATRIENE (2), URSOLIC ACID (3), ?-LUPEOL (4), ?-SITOSTEROL (5), 2-HYDROXY-3-(1,1-DIMETHYLALLYL)-1,4-NAPHTHOQUINONE (6), ?-DUNNIONE (7), VERBASCOSIDE (8), MARTYNOSIDE (9), AND SOME KNOWN MODEL COMPOUNDS PROVED TO BE INHIBITORS OF OXIDATION OF L-3,4-DIHYDROXYPHENYLALANINE (L-DOPA) CATALYZED BY TYROSINASE (EC 1.14.18.1) WITH AN IC50 BETWEEN 10.0 AND 200 PPM OR ?M, RESPECTIVELY, SUGGESTING THAT PHENOLIC MOIETIES IN THE MOLECULES ASSAYED ARE IMPORTANT FOR THE ACTIVITY.
Descripción
Palabras clave
VERBASCOSIDE, TYROSINASE INHIBITORY ACTIVITY, TRITERPENES, PROTEASE INHIBITION, PLANT?INSECT INTERACTION, PHENYLETHANOIDS, NAPHTHOQUINONES, IRIDOID MONOTERPENES, INSECT GROWTH INHIBITORY ACTIVITY, DITERPENES, DEFENSE MECHANISM, CALCEOLARIA TALCANA
