Artículos científicos

THIS STUDY CHARACTERIZED FIVE CRONOBACTER SPP. AND SIX SALMONELLA SPP. STRAINS THAT HAD BEEN ISOLATED FROM 155 SAMPLES OF POWDERED INFANT FORMULA (PIF) SOLD IN CHILE AND MANUFACTURED IN CHILE AND MEXICO IN 2018?2020. TWO STRAINS OF CRONOBACTER SAKAZAKII SEQUENCE TYPE (ST) ST1 AND ST31 (SEROTYPES O:1 AND O:2) AND ONE STRAIN OF CRONOBACTER MALONATICUS ST60 (O:1) WERE IDENTIFIED. ALL SALMONELLA STRAINS WERE IDENTIFIED AS SALMONELLA TYPHIMURIUM ST19 (SEROTYPE O:4) BY AVERAGE NUCLEOTIDE IDENTITY, RIBOSOMAL MULTILOCUS SEQUENCE TYPING (RMLST), AND CORE GENOME MLST (CGMLST). THE C. SAKAZAKII AND C. MALONATICUS ISOLATES WERE RESISTANT TO CEPHALOTHIN, WHEREAS THE SALMONELLA ISOLATES WERE RESISTANT TO OXACILLIN AND AMPICILLIN. NINETEEN ANTIBIOTIC RESISTANCE GENES WERE DETECTED IN THE C. SAKAZAKII AND C. MALONATICUS ISOLATES; THE MOST PREVALENT WERE MCR-9.1, BLACSA, AND BLACMA. IN SALMONELLA, 30 GENES ENCODING FOR AMINOGLYCOSIDE AND CEPHALOSPORIN RESISTANCE WERE IDENTIFIED, INCLUDING AAC(6?)-IAA, ?-LACTAMASES AMPH, AMPC1, AND MARA. IN THE CRONOBACTER ISOLATES, 32 VIRULENCE-ASSOCIATED GENES WERE DETECTED BY WGS AND CLUSTERED AS FLAGELLAR PROTEINS, OUTER MEMBRANE PROTEINS, CHEMOTAXIS, HEMOLYSINS, INVASION, PLASMINOGEN ACTIVATOR, COLONIZATION, TRANSCRIPTIONAL REGULATOR, SURVIVAL IN MACROPHAGES, USE OF SIALIC ACID, AND TOXIN-ANTITOXIN GENES. IN THE SALMONELLA STRAINS, 120 VIRULENCE ASSOCIATED GENES WERE DETECTED, ADHERENCE, MAGNESIUM UPTAKE, RESISTANCE TO ANTIMICROBIAL PEPTIDES, SECRETION SYSTEM, STRESS PROTEIN, TOXIN, RESISTANCE TO COMPLEMENT KILLING, AND EIGHT PATHOGENICITY ISLANDS. THE C. SAKAZAKII AND C. MALONATICUS STRAINS HARBORED I-E AND I-F CRISPR-CAS SYSTEMS AND CARRIED COL(PHHAD28) AND INCFIB(PCTU1) PLASMIDS, RESPECTIVELY. THE SALMONELLA STRAINS HARBORED TYPE I-E CRISPR-CAS SYSTEMS AND CARRIED INCFII(S) PLASMIDS. THE PRESENCE OF C. SAKAZAKII AND SALMONELLA IN PIF IS A HEALTH RISK FOR INFANTS AGED LESS THAN 6 MONTHS. FOR THIS REASON, SANITARY PRACTICES SHOULD BE REINFORCED

LISTERIA MONOCYTOGENES IS CAUSING LISTERIOSIS, A RARE BUT SEVERE FOODBORNE INFECTION. LISTERIOSIS AFFECTS PREGNANT WOMEN, NEWBORNS, OLDER ADULTS, AND IMMUNOCOMPROMISED INDIVIDUALS. READY-TO-EAT (RTE) FOODS ARE THE MOST COMMON SOURCES OF TRANSMISSION OF THE PATHOGEN THIS STUDY EXPLORED THE VIRULENCE FACTORS AND ANTIBIOTIC RESISTANCE IN L. MONOCYTOGENES STRAINS ISOLATED FROM READY-TO-EAT (RTE) FOODS THROUGH IN VITRO AND IN SILICO TESTING BY WHOLE-GENOME SEQUENCING (WGS). THE OVERALL POSITIVITY OF L. MONOCYTOGENES IN RTE FOOD SAMPLES WAS 3.1% AND 14 STRAINS WERE ISOLATED. L. MONOCYTOGENES ST8, ST2763, ST1, ST3, ST5, ST7, ST9, ST14, ST193, AND ST451 SEQUENCE TYPES WERE IDENTIFIED BY AVERAGE NUCLEOTIDE IDENTITY, RIBOSOMAL MULTILOCUS SEQUENCE TYPING (RMLST), AND CORE GENOME MLST. SEVEN ISOLATES HAD SEROTYPE 1/2A, FIVE 1/2B, ONE 4B, AND ONE 1/2C. THREE STRAINS EXHIBITED IN VITRO RESISTANCE TO AMPICILLIN AND 100% OF THE STRAINS CARRIED THE FOSX, LIN, NORB, MPRF, TETA, AND TETC RESISTANCE GENES. IN ADDITION, THE ARSBC, BCRBC, AND CLPL GENES WERE DETECTED, WHICH CONFERRED RESISTANCE TO STRESS AND DISINFECTANTS. ALL STRAINS HARBORED HLYA, PRFA, AND INLA GENES ALMOST THIRTY-TWO THE SHOWED THE BSH, CLPCEP, HLY, HPT, IAP/CWHA, INLA, INLB, IPEA, LSPA, MPL, PLCA, PCLB, OAT, PDGA, AND PRFA GENES. ONE ISOLATE EXHIBITED A TYPE 11 PREMATURE STOP CODON (PMSC) IN THE INLA GENE AND ANOTHER ISOLATE A NEW MUTATION (DELETION OF A IN POSITION 819). THE INC18(REP25), INC18(REP26), AND N1011A PLASMIDS AND MGES WERE FOUND IN NINE ISOLATES. TEN ISOLATES SHOWED CAS-TYPE II-B SYSTEMS; IN ADDITION, ANTI-CRISPR ACRIIA1 AND ACRIIA3 PHAGE-ASSOCIATED SYSTEMS WERE DETECTED IN THREE GENOMES. THESE VIRULENCE AND ANTIBIOTIC RESISTANCE TRAITS IN THE STRAINS ISOLATED IN THE RTE FOODS INDICATE A POTENTIAL PUBLIC HEALTH RISK FOR CONSUMERS.